Detailed information NTG0037430

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MESIL_RS06725
• Genome accession: NC_014212
• Coordinates: 1321456..1322595 (-)
• Length: 379 a.a.
• NCBI ID: WP_013157799.1
• Uniprot ID: D7BEI6
• Organism: Allomeiothermus silvanus DSM 9946
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1316456..1327595

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MESIL_RS06705 (Mesil_1332)	-	1316846..1319161 (-)	2316	WP_013157795.1	secretin N-terminal domain-containing protein	-
MESIL_RS06710 (Mesil_1333)	-	1319166..1320146 (-)	981	WP_013157796.1	hypothetical protein	-
MESIL_RS06715 (Mesil_1334)	-	1320143..1320838 (-)	696	WP_013157797.1	type 4a pilus biogenesis protein PilO	-
MESIL_RS06720 (Mesil_1335)	pilN	1320828..1321463 (-)	636	WP_013157798.1	hypothetical protein	Machinery gene
MESIL_RS06725 (Mesil_1336)	pilM	1321456..1322595 (-)	1140	WP_013157799.1	type IV pilus assembly protein PilM	Machinery gene
MESIL_RS06730 (Mesil_1337)	-	1323001..1324005 (-)	1005	WP_013157800.1	isocitrate/isopropylmalate dehydrogenase family protein	-
MESIL_RS06735 (Mesil_1338)	-	1324045..1324587 (-)	543	WP_013157801.1	DUF5317 domain-containing protein	-
MESIL_RS06740 (Mesil_1339)	-	1324605..1325996 (-)	1392	WP_013157802.1	HD-GYP domain-containing protein	-
MESIL_RS06745 (Mesil_1341)	-	1326549..1327367 (-)	819	WP_013157804.1	glycosyltransferase family 2 protein	-

Sequence

Protein

• Length: 379 a.a.
• Molecular weight: 41369.66 Da
• Isoelectric Point: 4.4828

>NTDB_id=37430 MESIL_RS06725 WP_013157799.1 1321456..1322595(-) (pilM) [Allomeiothermus silvanus DSM 9946]
MRELLSSLLRPRVEALGLEIGSANLKLVELSGYPPTLRGLAIRPTPPGTIQDGAIVEPGVLAGELRDMLGELRTRKRYVV
AAASNLAVITRTIQVPKMLPKQLEEAVRWEAERYIPFPIDEVVLDFAPLDDPETIADGEQMEVVVGAARQETVAGLVEAI
KAAGLEPLVIDVKPFAGLRPMEHQLKGENGQAPVTLFLEVGAESSALVLTRGERLLLNRIINLSGKDFTAAIGRAFNLDP
VAAEEAKKSYGLATIPTEDEELLLDFDAERERFNPARMYDAIRPVLVELTTELRRSLEFFRVQVGDLGIEQGFVAGGGSK
LRGLVPLLSDTLGIPLEPADPWQNISFDKNRFDGDYLRSLAPEFTVPLGLALRGVNPLD

Nucleotide

• Length: 1140 bp

>NTDB_id=37430 MESIL_RS06725 WP_013157799.1 1321456..1322595(-) (pilM) [Allomeiothermus silvanus DSM 9946]
GTGCGAGAGCTATTAAGCAGCTTGTTACGGCCACGGGTCGAGGCATTGGGCCTCGAGATCGGTTCGGCCAACCTCAAGCT
GGTGGAGCTATCCGGCTACCCTCCCACCCTCCGCGGTTTGGCCATCCGTCCTACACCGCCAGGCACCATCCAGGACGGAG
CCATCGTGGAGCCGGGGGTATTGGCCGGGGAGTTGCGCGATATGCTGGGCGAGTTACGCACCCGCAAACGCTACGTGGTG
GCTGCCGCCAGCAACCTGGCCGTGATCACCCGTACCATCCAGGTGCCCAAGATGCTGCCCAAGCAGCTCGAGGAGGCCGT
GCGCTGGGAAGCCGAGCGGTACATCCCTTTCCCCATTGACGAGGTGGTGCTGGACTTTGCTCCTTTGGATGATCCAGAGA
CCATCGCAGACGGGGAGCAGATGGAAGTAGTAGTCGGGGCAGCCCGCCAGGAAACGGTAGCGGGATTGGTTGAGGCCATC
AAGGCCGCGGGGCTCGAGCCCTTGGTCATCGATGTAAAACCTTTCGCCGGGCTCAGGCCGATGGAGCACCAGCTCAAAGG
CGAGAACGGGCAGGCCCCGGTAACGCTCTTCCTGGAGGTCGGGGCCGAGTCCAGCGCCCTAGTGCTAACCCGGGGCGAGC
GGCTCTTGCTAAACCGCATCATCAACCTCTCCGGCAAGGACTTCACCGCCGCCATCGGGCGGGCTTTCAACTTGGACCCA
GTGGCTGCCGAGGAAGCCAAGAAGAGCTACGGCCTGGCCACCATCCCCACCGAAGATGAAGAACTCCTCTTAGACTTCGA
TGCCGAGCGCGAGCGCTTCAACCCAGCCCGCATGTACGACGCCATTCGTCCGGTGCTGGTAGAGCTCACCACCGAGCTAA
GGCGTAGCCTGGAGTTCTTCCGGGTGCAAGTGGGCGACCTGGGCATCGAGCAGGGCTTTGTGGCCGGGGGGGGCAGCAAG
CTGCGCGGGCTGGTTCCGCTGCTATCGGACACCTTGGGAATCCCGCTCGAGCCCGCCGACCCCTGGCAAAACATCTCCTT
CGACAAAAACCGCTTTGATGGGGACTACCTGCGTAGCCTGGCCCCCGAGTTCACCGTCCCGCTAGGGCTGGCTTTACGGG
GGGTGAACCCGCTTGATTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (17-374)

3D structure

• AlphaFold DB: D7BEI6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Thermus thermophilus HB27	63.852	100	0.639