Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   FNS63_RS15585 Genome accession   NZ_CP041770
Coordinates   3162894..3163331 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain DH8030     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3157894..3168331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNS63_RS20260 (FNS63_15540) - 3158474..3158599 (-) 126 Protein_3016 hypothetical protein -
  FNS63_RS15550 (FNS63_15545) - 3158752..3159954 (+) 1203 WP_024084872.1 GTP-binding protein -
  FNS63_RS15555 (FNS63_15550) - 3160019..3161155 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  FNS63_RS15560 (FNS63_15555) - 3161170..3161604 (+) 435 WP_021494203.1 RDD family protein -
  FNS63_RS15565 (FNS63_15560) - 3161721..3161996 (+) 276 WP_369719109.1 YckD family protein -
  FNS63_RS20540 - 3162100..3162183 (+) 84 WP_223151977.1 family 1 glycosylhydrolase -
  FNS63_RS15575 (FNS63_15570) - 3162215..3162433 (+) 219 Protein_3022 family 1 glycosylhydrolase -
  FNS63_RS15580 (FNS63_15575) nin/comJ 3162474..3162872 (-) 399 WP_014304317.1 competence protein ComJ Regulator
  FNS63_RS15585 (FNS63_15580) nucA/comI 3162894..3163331 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  FNS63_RS15590 (FNS63_15585) hxlB 3163690..3164247 (-) 558 WP_003156587.1 6-phospho-3-hexuloisomerase -
  FNS63_RS15595 (FNS63_15590) hxlA 3164244..3164879 (-) 636 WP_024084873.1 3-hexulose-6-phosphate synthase -
  FNS63_RS15600 (FNS63_15595) - 3165111..3165473 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=374047 FNS63_RS15585 WP_003156588.1 3162894..3163331(-) (nucA/comI) [Bacillus amyloliquefaciens strain DH8030]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=374047 FNS63_RS15585 WP_003156588.1 3162894..3163331(-) (nucA/comI) [Bacillus amyloliquefaciens strain DH8030]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment