Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FO446_RS01225 Genome accession   NZ_CP041767
Coordinates   212621..213988 (+) Length   455 a.a.
NCBI ID   WP_088910268.1    Uniprot ID   A0A220MP74
Organism   Brevibacillus brevis strain B011     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 207621..218988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FO446_RS01205 (FO446_01210) - 207912..208376 (+) 465 WP_048035636.1 CtsR family transcriptional regulator -
  FO446_RS01210 (FO446_01215) - 208393..208911 (+) 519 WP_012683955.1 UvrB/UvrC motif-containing protein -
  FO446_RS01215 (FO446_01220) - 208931..210004 (+) 1074 WP_232774669.1 protein arginine kinase -
  FO446_RS01220 (FO446_01225) - 210065..212515 (+) 2451 WP_016742864.1 ATP-dependent Clp protease ATP-binding subunit -
  FO446_RS01225 (FO446_01230) radA 212621..213988 (+) 1368 WP_088910268.1 DNA repair protein RadA Machinery gene
  FO446_RS01230 (FO446_01235) disA 213997..215073 (+) 1077 WP_221869267.1 DNA integrity scanning diadenylate cyclase DisA -
  FO446_RS01235 (FO446_01240) pssA 215188..215916 (+) 729 WP_173612314.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  FO446_RS01240 (FO446_01245) - 216012..216407 (-) 396 WP_173612315.1 DUF1573 domain-containing protein -
  FO446_RS01245 (FO446_01250) - 216694..217782 (+) 1089 WP_173612316.1 PIN/TRAM domain-containing protein -
  FO446_RS01250 (FO446_01255) ispD 217810..218496 (+) 687 WP_232774670.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  FO446_RS01255 (FO446_01260) ispF 218500..218973 (+) 474 WP_232774671.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49427.78 Da        Isoelectric Point: 6.8250

>NTDB_id=373947 FO446_RS01225 WP_088910268.1 212621..213988(+) (radA) [Brevibacillus brevis strain B011]
MSKYKTKYACQECGYESPKWMGKCPGCSSWNTLVEEVTVKNAHRHEGLSGGQRQSAIPLTQVASEEEPRMDTTIGELNRV
LGGGLVPGSLILVGGDPGIGKSTLLLQTSFALAHQGAKVLYVSGEESAKQIKIRADRLHLQTPPMFVLAENDLDLIEQHI
NQVDPDVLIIDSIQTVFHPTIQSAAGSVAQVREATAQLMRIAKGKGIGTFIVGHVTKEGSIAGPRMLEHMVDAVLYFEGE
RHNTFRILRAVKNRFGSTNEIGIFEMKDRGLEEVANPSEIFLAERPFGVAGSTVVASMEGTRPVLVELQALVAPTSFVTP
RRTATGVDHQRVAMIMAVLEKRMGMMLQNQDAYVNVAGGVRLDEPAVDLAIAVSIASSFRDHATNPHDVVIGEIGLTGEV
RGVSRIEQRVREAHKLGFKRVIIPEKNIRGLDAPADIQVIGVSNIADALNEVIRR

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=373947 FO446_RS01225 WP_088910268.1 212621..213988(+) (radA) [Brevibacillus brevis strain B011]
ATGTCAAAGTATAAAACGAAATACGCGTGTCAAGAATGTGGCTATGAATCGCCAAAATGGATGGGGAAATGTCCAGGATG
CAGCAGCTGGAACACGTTGGTAGAGGAAGTGACGGTAAAAAATGCCCATCGTCACGAAGGGCTGAGCGGAGGCCAGCGCC
AATCTGCGATTCCATTAACCCAGGTAGCCAGTGAAGAAGAGCCACGGATGGATACGACGATTGGAGAATTGAATCGTGTT
CTAGGTGGAGGGCTTGTTCCGGGTTCACTCATTTTGGTGGGGGGAGATCCCGGAATCGGAAAGTCGACCTTGCTTTTGCA
GACTTCTTTTGCCTTGGCTCATCAAGGGGCGAAGGTTCTCTATGTATCGGGAGAGGAGTCGGCAAAACAAATCAAAATTC
GCGCGGATCGACTTCATTTGCAAACGCCGCCGATGTTTGTATTGGCAGAAAATGATTTGGATTTGATCGAACAGCATATT
AATCAAGTAGACCCGGATGTGCTGATTATTGACTCGATCCAGACCGTGTTTCACCCGACGATCCAATCGGCTGCGGGGAG
CGTCGCACAAGTGCGCGAGGCTACTGCTCAATTGATGCGAATTGCAAAAGGAAAAGGGATCGGTACCTTCATTGTCGGTC
ACGTAACCAAGGAAGGTTCTATCGCTGGTCCGCGTATGCTTGAGCACATGGTGGATGCTGTTCTCTATTTTGAAGGGGAG
CGCCATAATACGTTTCGAATTTTGCGCGCCGTCAAAAACCGTTTTGGTTCGACAAACGAAATCGGTATTTTCGAAATGAA
GGATCGCGGCTTGGAGGAAGTGGCGAATCCATCTGAGATATTTTTGGCAGAGCGTCCATTTGGCGTTGCGGGTTCCACGG
TCGTAGCCAGTATGGAGGGCACACGTCCCGTTTTGGTTGAGCTGCAAGCTCTCGTTGCCCCGACCAGCTTTGTAACCCCA
CGGAGAACGGCAACAGGGGTCGACCATCAGCGTGTGGCGATGATTATGGCAGTGTTGGAAAAGCGCATGGGCATGATGCT
GCAAAATCAGGATGCATATGTAAACGTAGCAGGTGGGGTCAGACTGGATGAGCCAGCAGTTGATTTGGCGATTGCCGTAA
GCATTGCAAGCAGCTTCCGTGACCACGCTACCAATCCGCATGATGTTGTTATCGGAGAAATTGGCTTGACTGGTGAGGTG
CGCGGTGTCTCTCGTATTGAGCAACGAGTACGAGAAGCACATAAATTGGGCTTTAAGCGTGTCATCATACCAGAGAAAAA
CATCCGCGGTCTGGATGCCCCAGCAGATATTCAAGTAATTGGTGTGAGCAATATTGCCGATGCGTTGAACGAGGTGATAA
GGAGGTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-219)

Subunit ChlI of Mg-chelatase (ChlI)

(329-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A220MP74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.929

99.341

0.655
  radA Streptococcus pneumoniae Rx1

58.057

99.56

0.578
  radA Streptococcus pneumoniae D39

58.057

99.56

0.578
  radA Streptococcus pneumoniae R6

58.057

99.56

0.578
  radA Streptococcus pneumoniae TIGR4

58.057

99.56

0.578
  radA Streptococcus mitis NCTC 12261

58.057

99.56

0.578
  radA Streptococcus mitis SK321

58.057

99.56

0.578

Multiple sequence alignment