Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M301_RS07555 Genome accession   NC_014207
Coordinates   1645295..1646389 (+) Length   364 a.a.
NCBI ID   WP_013148174.1    Uniprot ID   D7DIH7
Organism   Methylotenera versatilis 301     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1640295..1651389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M301_RS07535 (M301_1478) - 1640780..1641244 (+) 465 WP_013148172.1 YhcH/YjgK/YiaL family protein -
  M301_RS07540 mnmD 1641258..1641965 (+) 708 WP_041359382.1 tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD -
  M301_RS07545 mnmC 1642097..1643323 (+) 1227 WP_049769961.1 FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC -
  M301_RS07550 (M301_1481) - 1643333..1645222 (+) 1890 WP_013148173.1 tetratricopeptide repeat protein -
  M301_RS07555 (M301_1482) pilU 1645295..1646389 (+) 1095 WP_013148174.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M301_RS07560 (M301_1483) - 1646402..1647307 (-) 906 WP_013148175.1 DMT family transporter -
  M301_RS07565 (M301_1484) dapC 1647435..1648613 (+) 1179 WP_013148176.1 succinyldiaminopimelate transaminase -
  M301_RS07570 (M301_1485) miaA 1648740..1649681 (-) 942 WP_013148177.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  M301_RS07575 (M301_1486) - 1649693..1650289 (-) 597 WP_013148178.1 DUF6988 family protein -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40043.20 Da        Isoelectric Point: 6.1085

>NTDB_id=37388 M301_RS07555 WP_013148174.1 1645295..1646389(+) (pilU) [Methylotenera versatilis 301]
MAAIDISPILKFMLDKGGSDLFFSTGAIIHIEVEGDTLPINNQIMTPGMIKEIAYSMMTADQITEFESTLECNFAISKKD
VGRFRVNVFKQRGEVGMVIRHIKTDIPTIESLGLPAILKDLILRKRGLILVVGATGSGKTTTLASMIDHRNATTNGHILT
LEDPIEFIHPHKKSVVDQREVGIDTLSFENGLKNAMRQAPDVILIGEVRDMEGMKNAMAYAETGHLCLATLHANNANQAL
ERIISFFPEDARAGLLLGLSMNLVSIVSQRLIPGKLSKRVAATEVMINTPYISELIQKQKMGDMKDTMADSNDIGMNTFD
QSLFRLFSNGKIDEENALSNADSRNDLSLKIRFAAEGNRNTSFG

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=37388 M301_RS07555 WP_013148174.1 1645295..1646389(+) (pilU) [Methylotenera versatilis 301]
ATGGCCGCAATCGACATCAGTCCAATCTTAAAGTTCATGCTAGATAAAGGTGGCTCCGACCTCTTTTTTAGTACGGGCGC
AATCATTCATATTGAAGTTGAAGGCGACACACTGCCTATCAACAACCAAATAATGACGCCTGGCATGATTAAAGAAATCG
CTTATTCGATGATGACTGCAGATCAAATCACGGAGTTTGAATCGACTTTAGAATGTAATTTTGCTATTAGCAAGAAAGAT
GTCGGGCGTTTCCGCGTAAATGTATTCAAGCAACGCGGTGAAGTGGGCATGGTAATACGTCATATTAAAACGGATATCCC
AACAATTGAATCTTTAGGGCTTCCTGCGATATTAAAAGATCTAATTTTACGTAAGCGCGGCTTAATTCTAGTGGTTGGTG
CAACGGGTTCAGGCAAAACCACCACCCTCGCCTCTATGATTGATCATCGTAACGCAACGACTAACGGTCACATTCTGACT
CTTGAAGACCCGATTGAGTTTATTCACCCGCATAAAAAATCGGTTGTTGATCAACGCGAAGTTGGTATTGATACACTCTC
TTTTGAAAACGGTCTTAAAAATGCCATGCGCCAAGCGCCAGATGTGATTTTAATTGGGGAAGTCCGTGATATGGAAGGCA
TGAAAAATGCCATGGCTTATGCAGAAACGGGCCATTTGTGCCTTGCTACATTGCATGCCAACAATGCCAACCAAGCGCTT
GAGCGCATCATTTCATTCTTCCCAGAAGACGCCAGAGCGGGCTTACTACTAGGCTTATCAATGAATTTGGTGTCTATTGT
TTCTCAACGCCTTATTCCAGGTAAACTCAGCAAGCGTGTAGCCGCGACTGAAGTCATGATTAATACGCCGTACATTTCAG
AGCTGATTCAAAAGCAAAAAATGGGTGATATGAAAGATACTATGGCTGACAGCAACGACATCGGCATGAACACTTTTGAC
CAATCGTTATTCAGGTTGTTTAGCAACGGTAAAATTGATGAGGAGAATGCTCTGTCAAATGCAGATTCTCGCAACGATTT
ATCACTAAAAATTCGCTTTGCTGCTGAAGGTAATCGTAACACTAGCTTTGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(56-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D7DIH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.52

97.253

0.53
  pilU Acinetobacter baylyi ADP1

49.721

98.352

0.489
  pilU Vibrio cholerae strain A1552

49.714

96.154

0.478
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.695

93.681

0.409
  pilT Pseudomonas aeruginosa PAK

42.308

92.857

0.393
  pilT Acinetobacter baylyi ADP1

41.176

93.407

0.385
  pilT Legionella pneumophila strain ERS1305867

41.617

91.758

0.382
  pilT Legionella pneumophila strain Lp02

41.617

91.758

0.382
  pilT Acinetobacter nosocomialis M2

40.828

92.857

0.379
  pilT Pseudomonas stutzeri DSM 10701

40.828

92.857

0.379
  pilT Acinetobacter baumannii strain A118

40.533

92.857

0.376
  pilT Acinetobacter baumannii D1279779

40.533

92.857

0.376
  pilT Neisseria gonorrhoeae MS11

38.04

95.33

0.363
  pilT Neisseria meningitidis 8013

38.04

95.33

0.363

Multiple sequence alignment