Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M301_RS07285 Genome accession   NC_014207
Coordinates   1585749..1587122 (-) Length   457 a.a.
NCBI ID   WP_013148125.1    Uniprot ID   D7DIC8
Organism   Methylotenera versatilis 301     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1580749..1592122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M301_RS07255 (M301_1424) - 1580891..1581529 (+) 639 WP_013148119.1 helix-turn-helix domain-containing protein -
  M301_RS07260 (M301_1425) - 1581544..1581999 (+) 456 WP_013148120.1 GNAT family N-acetyltransferase -
  M301_RS07265 (M301_1426) - 1582062..1582415 (-) 354 WP_013148121.1 cupin domain-containing protein -
  M301_RS07270 (M301_1427) hpnC 1582622..1583524 (+) 903 WP_013148122.1 squalene synthase HpnC -
  M301_RS07275 (M301_1428) hpnD 1583533..1584369 (+) 837 WP_013148123.1 presqualene diphosphate synthase HpnD -
  M301_RS07280 (M301_1429) hpnE 1584370..1585719 (+) 1350 WP_013148124.1 hydroxysqualene dehydroxylase HpnE -
  M301_RS07285 (M301_1430) radA 1585749..1587122 (-) 1374 WP_013148125.1 DNA repair protein RadA Machinery gene
  M301_RS07290 (M301_1431) dnaB 1587131..1588513 (-) 1383 WP_013148126.1 replicative DNA helicase -
  M301_RS07295 (M301_1432) rplI 1588717..1589166 (-) 450 WP_013148127.1 50S ribosomal protein L9 -
  M301_RS07300 (M301_1433) rpsR 1589179..1589409 (-) 231 WP_013148128.1 30S ribosomal protein S18 -
  M301_RS07305 (M301_1434) priB 1589446..1589748 (-) 303 WP_013148129.1 primosomal replication protein N -
  M301_RS07310 (M301_1435) rpsF 1589761..1590159 (-) 399 WP_013148130.1 30S ribosomal protein S6 -
  M301_RS07315 (M301_1436) - 1590338..1590799 (-) 462 WP_013148131.1 DUF2721 domain-containing protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 48775.36 Da        Isoelectric Point: 7.1834

>NTDB_id=37387 M301_RS07285 WP_013148125.1 1585749..1587122(-) (radA) [Methylotenera versatilis 301]
MAKAKTVYSCTECGASEPKWQGQCPSCMAWNTLVESVEESGTSTNRYANKFEGLAQSSTLQKISSIKAADIARKPTGISE
FDRVLGGGLVEGGVVLIGGDPGIGKSTLLLQVLCHLGKSSQAIYVSGEESPQQIAMRAKRLGLDASEVELLAEINLEKIL
ATLQTHKPNIAVIDSIQTVYSEALQSAPGSVAQVRECSAQLTRIAKQLGITVILVGHVTKEGSLAGPRVLEHIVDTVLYF
EGDQNSSFRLIRAFKNRFGAVNELGVFAMTEHGLREVSNPSALFLSHHEGEVAGSCITVTMEGTRPLLIEIQALVDESHA
PSPKRLCVGLEQNRLAMLLAVLHRHVGIPCFDQDVFINAVGGVKIAEPAVDLAVILSIVSSLKNKPLDNKLIVFGEVGLA
GEVRPVQGGQVRLKEAAKLGFTKAIVPKANAPKAKIAGMEVIAVERLEQALNQLRNG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=37387 M301_RS07285 WP_013148125.1 1585749..1587122(-) (radA) [Methylotenera versatilis 301]
GTGGCAAAAGCTAAAACCGTATACAGCTGCACAGAATGTGGCGCCAGCGAGCCTAAGTGGCAAGGTCAATGCCCAAGCTG
CATGGCTTGGAACACGCTGGTTGAAAGCGTAGAAGAAAGCGGCACGTCGACCAATCGCTATGCTAATAAGTTTGAAGGTT
TAGCGCAAAGTAGTACTTTGCAAAAGATAAGTAGCATCAAAGCTGCTGATATTGCACGCAAACCAACAGGAATCAGCGAG
TTTGACCGTGTGTTGGGTGGCGGTCTAGTTGAAGGTGGTGTGGTTTTGATCGGCGGTGATCCTGGTATCGGTAAATCGAC
TTTATTGCTACAGGTTTTGTGTCATTTAGGAAAGTCCAGCCAAGCCATTTATGTCAGTGGCGAAGAATCCCCGCAGCAAA
TCGCCATGCGCGCCAAGCGCTTAGGGCTTGATGCAAGTGAAGTTGAGCTTTTAGCCGAAATTAATCTAGAGAAAATTTTA
GCAACGCTTCAAACGCATAAACCTAATATCGCGGTCATTGATTCTATTCAAACCGTCTATTCCGAAGCTTTACAATCCGC
GCCAGGTTCAGTGGCGCAGGTGCGTGAATGCTCTGCGCAATTGACCAGAATTGCTAAACAACTAGGCATTACAGTGATAT
TGGTAGGGCATGTTACCAAAGAGGGCTCATTGGCAGGACCGCGAGTGCTAGAGCATATTGTTGATACCGTTTTGTATTTT
GAAGGTGATCAGAATTCTAGTTTTAGACTAATTCGTGCATTCAAAAATCGTTTCGGTGCAGTCAATGAGCTGGGCGTGTT
TGCCATGACTGAGCATGGTTTGCGTGAAGTTTCTAATCCATCAGCTTTATTTTTATCGCATCATGAAGGTGAAGTGGCGG
GTAGCTGTATTACTGTGACGATGGAAGGTACGCGCCCATTATTGATTGAAATTCAGGCTTTGGTTGATGAAAGCCACGCG
CCTAGCCCCAAAAGATTGTGTGTTGGTTTAGAGCAAAATCGTTTAGCGATGTTGTTAGCCGTTTTGCATCGTCATGTTGG
CATTCCGTGTTTTGATCAAGACGTCTTTATAAACGCTGTGGGTGGTGTCAAAATTGCTGAGCCTGCGGTCGATTTAGCGG
TGATACTTTCAATCGTTTCTTCTTTAAAAAACAAACCATTAGATAACAAACTTATAGTGTTTGGTGAGGTTGGTTTGGCA
GGTGAAGTGCGCCCAGTGCAAGGTGGTCAGGTGCGTTTGAAAGAAGCTGCGAAATTGGGTTTTACTAAAGCAATTGTACC
TAAAGCGAATGCGCCAAAAGCCAAGATTGCCGGTATGGAAGTGATTGCGGTAGAGCGCCTAGAACAAGCTTTGAACCAAC
TCAGAAATGGTTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(352-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D7DIC8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.877

99.781

0.508
  radA Streptococcus mitis SK321

46.725

100

0.468
  radA Streptococcus mitis NCTC 12261

46.507

100

0.466
  radA Streptococcus pneumoniae TIGR4

46.593

99.562

0.464
  radA Streptococcus pneumoniae D39

46.593

99.562

0.464
  radA Streptococcus pneumoniae Rx1

46.593

99.562

0.464
  radA Streptococcus pneumoniae R6

46.593

99.562

0.464

Multiple sequence alignment