Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G6Q95_RS00515 Genome accession   NZ_CP049348
Coordinates   117400..117840 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain 3R     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 112400..122840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6Q95_RS00495 coaE 112890..113510 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  G6Q95_RS25425 - 113535..113579 (+) 45 WP_120795372.1 protein YacM -
  G6Q95_RS00500 guaC 113735..114778 (+) 1044 WP_001217338.1 GMP reductase -
  G6Q95_RS00505 hofC 114813..116015 (-) 1203 WP_000157234.1 protein transport protein HofC -
  G6Q95_RS00510 pilB 116005..117390 (-) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  G6Q95_RS00515 pilA 117400..117840 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  G6Q95_RS00520 nadC 118043..118936 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G6Q95_RS00525 ampD 119024..119575 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G6Q95_RS00530 ampE 119572..120426 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  G6Q95_RS00535 aroP 120469..121842 (-) 1374 WP_001331235.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=373727 G6Q95_RS00515 WP_000360895.1 117400..117840(-) (pilA) [Escherichia coli strain 3R]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=373727 G6Q95_RS00515 WP_000360895.1 117400..117840(-) (pilA) [Escherichia coli strain 3R]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment