Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G6Z93_RS18790 Genome accession   NZ_CP049204
Coordinates   3951628..3952068 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain PapM-32-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3946628..3957068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6Z93_RS18770 (G6Z93_18770) aroP 3947626..3948999 (+) 1374 WP_075842203.1 aromatic amino acid transporter AroP -
  G6Z93_RS18775 (G6Z93_18775) ampE 3949042..3949896 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  G6Z93_RS18780 (G6Z93_18780) ampD 3949893..3950444 (-) 552 WP_097397835.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G6Z93_RS18785 (G6Z93_18785) nadC 3950532..3951425 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G6Z93_RS18790 (G6Z93_18790) pilA 3951628..3952068 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  G6Z93_RS18795 (G6Z93_18795) pilB 3952078..3953463 (+) 1386 WP_112924886.1 type II secretion system protein GspE Machinery gene
  G6Z93_RS18800 (G6Z93_18800) hofC 3953453..3954655 (+) 1203 WP_097470404.1 protein transport protein HofC -
  G6Z93_RS18805 (G6Z93_18805) guaC 3954690..3955733 (-) 1044 WP_032222088.1 GMP reductase -
  G6Z93_RS23060 - 3955889..3955933 (-) 45 WP_120795372.1 protein YacM -
  G6Z93_RS18810 (G6Z93_18810) coaE 3955958..3956578 (+) 621 WP_001269510.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=373288 G6Z93_RS18790 WP_000360904.1 3951628..3952068(+) (pilA) [Escherichia coli strain PapM-32-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=373288 G6Z93_RS18790 WP_000360904.1 3951628..3952068(+) (pilA) [Escherichia coli strain PapM-32-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363