Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BMB171_RS19875 Genome accession   NC_014171
Coordinates   3902991..3903425 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis BMB171     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3897991..3908425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMB171_RS19855 (BMB171_C3666) - 3899166..3899723 (+) 558 WP_000062061.1 PadR family transcriptional regulator -
  BMB171_RS19865 (BMB171_C3667) metE 3900237..3902525 (+) 2289 WP_001007608.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BMB171_RS19870 (BMB171_C3668) comJ 3902564..3902954 (-) 391 Protein_3867 competence protein ComJ -
  BMB171_RS19875 (BMB171_C3669) nucA/comI 3902991..3903425 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  BMB171_RS19880 (BMB171_C3671) - 3903652..3904401 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  BMB171_RS19885 (BMB171_C3672) kinB 3904440..3905714 (-) 1275 WP_000420370.1 sporulation sensor histidine kinase KinB -
  BMB171_RS19890 (BMB171_C3673) - 3906025..3906225 (+) 201 WP_000929278.1 hypothetical protein -
  BMB171_RS19895 (BMB171_C3674) - 3906348..3907511 (+) 1164 WP_000434582.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=37309 BMB171_RS19875 WP_000811494.1 3902991..3903425(-) (nucA/comI) [Bacillus thuringiensis BMB171]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=37309 BMB171_RS19875 WP_000811494.1 3902991..3903425(-) (nucA/comI) [Bacillus thuringiensis BMB171]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGTTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment