Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G5T40_RS23870 Genome accession   NZ_CP049196
Coordinates   5016385..5017881 (+) Length   498 a.a.
NCBI ID   WP_001040615.1    Uniprot ID   -
Organism   Escherichia coli strain E118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5011385..5022881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5T40_RS23840 tusA 5011631..5011876 (+) 246 WP_000130615.1 sulfurtransferase TusA -
  G5T40_RS23845 zntA 5011979..5014177 (-) 2199 WP_001521507.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  G5T40_RS23850 yhhN 5014251..5014877 (-) 627 WP_000964718.1 lysoplasmalogenase -
  G5T40_RS23855 yhhM 5015018..5015377 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  G5T40_RS23860 yhhL 5015380..5015649 (-) 270 WP_001295207.1 DUF1145 family protein -
  G5T40_RS23865 rsmD 5015639..5016235 (-) 597 WP_001521506.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  G5T40_RS23870 pilA 5016385..5017881 (+) 1497 WP_001040615.1 signal recognition particle-docking protein FtsY Machinery gene
  G5T40_RS23875 ftsE 5017884..5018552 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  G5T40_RS23880 ftsX 5018545..5019603 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  G5T40_RS23885 rpoH 5019848..5020702 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  G5T40_RS23890 livJ 5020974..5022077 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  G5T40_RS23895 panM 5022277..5022660 (-) 384 WP_000778790.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54649.46 Da        Isoelectric Point: 4.1639

>NTDB_id=372967 G5T40_RS23870 WP_001040615.1 5016385..5017881(+) (pilA) [Escherichia coli strain E118]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEPAKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=372967 G5T40_RS23870 WP_001040615.1 5016385..5017881(+) (pilA) [Escherichia coli strain E118]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCCGGCGCAAGAGCCTGCGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTTGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTAGCTAT
CGAGCGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGATGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTTGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACTGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378