Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G6G93_RS09035 Genome accession   NZ_CP049118
Coordinates   1845106..1846077 (-) Length   323 a.a.
NCBI ID   WP_072222310.1    Uniprot ID   -
Organism   Escherichia coli strain EC931     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1840106..1851077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6G93_RS09005 (G6G93_09060) yggI 1840771..1841268 (+) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -
  G6G93_RS09010 (G6G93_09065) endA 1841363..1842070 (+) 708 WP_000286500.1 deoxyribonuclease I -
  G6G93_RS09015 (G6G93_09070) rsmE 1842150..1842881 (+) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G6G93_RS09020 (G6G93_09075) gshB 1842894..1843844 (+) 951 WP_000593259.1 glutathione synthase -
  G6G93_RS09025 (G6G93_09080) yqgE 1843953..1844516 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  G6G93_RS09030 (G6G93_09085) ruvX 1844516..1844932 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  G6G93_RS09035 (G6G93_09090) pilT 1845106..1846077 (-) 972 WP_072222310.1 type IV pilus twitching motility protein PilT Machinery gene
  G6G93_RS09040 (G6G93_09095) yggS 1846095..1846799 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  G6G93_RS09045 (G6G93_09100) yggT 1846817..1847383 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  G6G93_RS09050 (G6G93_09105) yggU 1847380..1847670 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  G6G93_RS09055 (G6G93_09110) rdgB 1847678..1848271 (+) 594 WP_001174737.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  G6G93_RS09060 (G6G93_09115) hemW 1848264..1849400 (+) 1137 WP_058905404.1 radical SAM family heme chaperone HemW -
  G6G93_RS09065 (G6G93_09120) yggM 1849465..1850472 (-) 1008 WP_000745230.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 35788.98 Da        Isoelectric Point: 5.9975

>NTDB_id=372640 G6G93_RS09035 WP_072222310.1 1845106..1846077(-) (pilT) [Escherichia coli strain EC931]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILATGSGKSTTLAAMVGYLNQHADAHILTLEDPVEYLY
TSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDSFPA
QEKDPVRSQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKE
GRL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=372640 G6G93_RS09035 WP_072222310.1 1845106..1846077(-) (pilT) [Escherichia coli strain EC931]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGCGACGGGGAGCGGCAAATCTACCACGC
TGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAATATCTCTAT
ACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCGGGCTGCATT
GCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGGCGGCAGAAA
CCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCGTTTCCGGCG
CAGGAAAAAGATCCCGTGCGTAGTCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGTGGATAAACA
GGAAGGACGCGTGGCGTTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGAAAACCCACC
AGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGGGTGAAAGAA
GGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.495
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.495
  pilT Neisseria meningitidis 8013

48.171

100

0.489
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.486
  pilT Acinetobacter baylyi ADP1

45.872

100

0.464
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.464
  pilT Acinetobacter baumannii D1279779

45.566

100

0.461
  pilT Acinetobacter nosocomialis M2

45.566

100

0.461
  pilT Acinetobacter baumannii strain A118

45.566

100

0.461
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.461
  pilT Legionella pneumophila strain Lp02

44.648

100

0.452
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.452
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.195

98.452

0.406
  pilU Vibrio cholerae strain A1552

38.602

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.381
  pilU Acinetobacter baylyi ADP1

36.646

99.69

0.365

Multiple sequence alignment