Detailed information NTG0372640

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: FNL97_RS17305
• Genome accession: NZ_CP041632
• Coordinates: 3373026..3374399 (-)
• Length: 457 a.a.
• NCBI ID: WP_012748858.1
• Uniprot ID: A0A150N8M8
• Organism: Geobacillus sp. E263
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 3368026..3379399

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FNL97_RS17280	cysE	3368027..3368698 (-)	672	WP_143416242.1	serine O-acetyltransferase	-
FNL97_RS17285	gltX	3369042..3370502 (-)	1461	WP_062677078.1	glutamate--tRNA ligase	-
FNL97_RS17290	ispF	3370617..3371093 (-)	477	WP_062677077.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
FNL97_RS17295	ispD	3371110..3371796 (-)	687	WP_012748860.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
FNL97_RS17300	-	3371820..3372914 (-)	1095	WP_012748859.1	PIN/TRAM domain-containing protein	-
FNL97_RS17305	radA	3373026..3374399 (-)	1374	WP_012748858.1	DNA repair protein RadA	Machinery gene
FNL97_RS17310	clpC	3374486..3376921 (-)	2436	WP_143416243.1	ATP-dependent protease ATP-binding subunit ClpC	-
FNL97_RS17315	-	3376921..3378009 (-)	1089	WP_143416244.1	protein arginine kinase	-
FNL97_RS17320	-	3378014..3378562 (-)	549	WP_012748855.1	UvrB/UvrC motif-containing protein	-
FNL97_RS17325	-	3378576..3379037 (-)	462	WP_143416245.1	CtsR family transcriptional regulator	-

Sequence

Protein

• Length: 457 a.a.
• Molecular weight: 49948.62 Da
• Isoelectric Point: 7.4850

>NTDB_id=372640 FNL97_RS17305 WP_012748858.1 3373026..3374399(-) (radA) [Geobacillus sp. E263]
MGKKKTKFVCQECGYESAKWLGRCPGCSTWNSMVEEIERTKPMTRGVFVHSTDDIISKPVPITTVTTTQEPRIQTNISEF
NRVLGGGIVKGSLVLIGGDPGIGKSTLLLQVSAQLAAMQHKVLYISGEESVKQTKLRADRLHIASDQLYVLAETDLEYIA
EAIENVRPACVVIDSIQTIYRADITSAPGSVSQVRECTAELMKIAKTKGIAIFIVGHVTKEGAIAGPRILEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEIGIFEMRDSGLIEVENPSEVFLEERSRGVAGSTVVAAMEGTRPVLVEIQALVSPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDQPTNPSDVIIGEVGLT
GEVRRVSRIEQRVQEAVKLGFSRIIIPKNNLAGWNPPKHITVIGVSHVAEALEHTLQ

Nucleotide

• Length: 1374 bp

>NTDB_id=372640 FNL97_RS17305 WP_012748858.1 3373026..3374399(-) (radA) [Geobacillus sp. E263]
ATGGGGAAAAAGAAAACAAAGTTTGTTTGTCAAGAATGCGGTTATGAATCAGCGAAATGGCTTGGAAGATGTCCAGGATG
CAGTACATGGAATTCGATGGTCGAGGAAATTGAGCGGACAAAGCCAATGACAAGAGGAGTGTTTGTGCATTCCACGGATG
ATATCATCTCAAAACCAGTTCCAATTACTACCGTGACCACAACGCAAGAGCCGCGCATACAAACGAATATTTCTGAATTT
AACCGCGTATTAGGTGGGGGAATTGTGAAAGGTTCGCTCGTTTTAATCGGTGGGGATCCAGGCATTGGAAAATCAACGCT
TCTTTTGCAAGTTTCTGCGCAACTTGCTGCTATGCAACATAAAGTATTATATATATCCGGCGAAGAATCCGTAAAACAAA
CGAAATTGCGCGCTGACCGCCTTCACATTGCTTCCGACCAATTGTATGTATTAGCGGAGACAGATTTGGAATATATTGCA
GAAGCAATCGAAAATGTTCGCCCCGCTTGTGTTGTGATTGATTCGATTCAAACGATTTACCGTGCAGATATTACGTCCGC
ACCAGGAAGTGTATCGCAAGTCCGTGAATGTACAGCGGAACTGATGAAAATTGCCAAAACAAAAGGAATTGCCATTTTTA
TTGTCGGCCATGTAACGAAAGAGGGAGCGATTGCCGGTCCAAGAATTTTAGAGCATATGGTGGATACAGTTCTTTATTTC
GAAGGAGAACGTCACCATACGTATCGTATTTTACGGGCGGTGAAAAACCGTTTCGGCTCCACAAATGAAATTGGTATTTT
TGAAATGAGAGATTCAGGACTTATCGAGGTCGAAAACCCTTCAGAAGTGTTTTTAGAAGAACGTTCCCGAGGGGTGGCAG
GTTCAACCGTTGTCGCGGCAATGGAAGGAACTCGTCCTGTATTAGTCGAAATTCAAGCATTAGTATCTCCAACGAGTTTT
GGCAACCCGCGGCGGATGGCAACAGGAATCGACCATAACCGTGTTTCTTTATTAATGGCCGTATTGGAAAAAAGGGTCGG
CCTGCTTTTACAAAATCAGGACGCTTACTTGAAAGTGGCAGGGGGAGTAAAATTAGACGAGCCAGCGATTGATTTGGCGA
TTGCCGTAAGCATTGCCTCAAGTTTCCGCGATCAGCCAACCAACCCGTCCGATGTGATTATCGGCGAGGTCGGTTTAACT
GGGGAAGTACGCCGTGTTTCCCGTATTGAACAACGGGTGCAAGAAGCAGTAAAATTAGGGTTTTCTCGCATCATTATTCC
TAAAAACAATTTAGCAGGATGGAATCCGCCAAAACATATAACCGTGATCGGTGTGTCCCACGTTGCCGAGGCACTTGAAC
ATACGTTGCAGTAA

Domains

Predicted by InterproScan.

• Subunit ChlI of Mg-chelatase (ChlI) (365-430)

• AAA domain (AAA_25) (68-222)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

3D structure

• AlphaFold DB: A0A150N8M8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	76.586	100	0.766
radA	Streptococcus mitis NCTC 12261	63.596	99.781	0.635
radA	Streptococcus mitis SK321	63.377	99.781	0.632
radA	Streptococcus pneumoniae Rx1	63.377	99.781	0.632
radA	Streptococcus pneumoniae D39	63.377	99.781	0.632
radA	Streptococcus pneumoniae R6	63.377	99.781	0.632
radA	Streptococcus pneumoniae TIGR4	63.377	99.781	0.632