Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   G5V60_RS09360 Genome accession   NZ_CP049101
Coordinates   1969423..1970850 (-) Length   475 a.a.
NCBI ID   WP_000980557.1    Uniprot ID   -
Organism   Escherichia coli strain EC28     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1964423..1975850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5V60_RS09325 (G5V60_09315) hisL 1965007..1965057 (-) 51 WP_001364200.1 his operon leader peptide -
  G5V60_RS09330 (G5V60_09320) yefM 1965340..1965591 (+) 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  G5V60_RS09335 (G5V60_09325) yoeB 1965588..1965842 (+) 255 WP_164699364.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  G5V60_RS09340 (G5V60_09330) yeeZ 1965925..1966749 (+) 825 WP_000754740.1 SDR family oxidoreductase -
  G5V60_RS09345 (G5V60_09335) yeeY 1966795..1967724 (+) 930 WP_045133079.1 LysR substrate-binding domain-containing protein -
  G5V60_RS09350 (G5V60_09340) yoeI 1967939..1968001 (+) 63 WP_010723108.1 membrane protein YoeI -
  G5V60_RS09355 (G5V60_09345) plaP 1967991..1969349 (+) 1359 WP_164699365.1 putrescine/proton symporter PlaP -
  G5V60_RS09360 (G5V60_09350) sbcB 1969423..1970850 (-) 1428 WP_000980557.1 exodeoxyribonuclease I Machinery gene
  G5V60_RS09365 (G5V60_09355) dacD 1971059..1972225 (+) 1167 WP_045133078.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  G5V60_RS09370 (G5V60_09360) sbmC 1972344..1972817 (+) 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
  G5V60_RS09375 (G5V60_09365) yeeA 1973015..1974073 (+) 1059 WP_001200890.1 FUSC family protein -
  G5V60_RS09380 (G5V60_09370) tmaR 1974245..1974574 (+) 330 WP_000450409.1 DUF496 family protein -
  G5V60_RS09385 (G5V60_09375) - 1974674..1975117 (-) 444 WP_001189438.1 EutP/PduV family microcompartment system protein -
  G5V60_RS09390 (G5V60_09380) pduU 1975122..1975472 (-) 351 WP_000211397.1 propanediol utilization microcompartment protein PduU -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54455.73 Da        Isoelectric Point: 5.4700

>NTDB_id=372406 G5V60_RS09360 WP_000980557.1 1969423..1970850(-) (sbcB) [Escherichia coli strain EC28]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=372406 G5V60_RS09360 WP_000980557.1 1969423..1970850(-) (sbcB) [Escherichia coli strain EC28]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAGGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTATGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(212-472)

Exonuclease (RNase_T)

(13-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583

Multiple sequence alignment