Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G5V60_RS01415 Genome accession   NZ_CP049101
Coordinates   305331..306827 (+) Length   498 a.a.
NCBI ID   WP_097478857.1    Uniprot ID   -
Organism   Escherichia coli strain EC28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 300331..311827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5V60_RS01385 (G5V60_01385) tusA 300578..300823 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  G5V60_RS01390 (G5V60_01390) zntA 300925..303123 (-) 2199 WP_000106599.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  G5V60_RS01395 (G5V60_01395) yhhN 303197..303823 (-) 627 WP_000964718.1 lysoplasmalogenase -
  G5V60_RS01400 (G5V60_01400) yhhM 303964..304323 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  G5V60_RS01405 (G5V60_01405) yhhL 304326..304595 (-) 270 WP_001295207.1 DUF1145 family protein -
  G5V60_RS01410 (G5V60_01410) rsmD 304585..305181 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  G5V60_RS01415 (G5V60_01415) pilA 305331..306827 (+) 1497 WP_097478857.1 signal recognition particle-docking protein FtsY Machinery gene
  G5V60_RS01420 (G5V60_01420) ftsE 306830..307498 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  G5V60_RS01425 (G5V60_01425) ftsX 307491..308549 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  G5V60_RS01430 (G5V60_01430) rpoH 308794..309648 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  G5V60_RS01435 (G5V60_01435) livJ 309919..311022 (+) 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  G5V60_RS01440 (G5V60_01440) ataR 311171..311437 (+) 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54652.46 Da        Isoelectric Point: 4.1733

>NTDB_id=372375 G5V60_RS01415 WP_097478857.1 305331..306827(+) (pilA) [Escherichia coli strain EC28]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEVETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=372375 G5V60_RS01415 WP_097478857.1 305331..306827(+) (pilA) [Escherichia coli strain EC28]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGTGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACAGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGATGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACCATCGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment