Detailed information NTG0372373

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: G5V60_RS00970
• Genome accession: NZ_CP049101
• Coordinates: 204491..205474 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196477.1
• Uniprot ID: A0A0H2VBW7
• Organism: Escherichia coli strain EC28
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 199491..210474

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G5V60_RS00955 (G5V60_00955)	dppA	200635..202242 (+)	1608	WP_001222871.1	dipeptide ABC transporter substrate-binding protein DppA	-
G5V60_RS00960 (G5V60_00960)	dppB	202549..203568 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
G5V60_RS00965 (G5V60_00965)	dppC	203578..204480 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
G5V60_RS00970 (G5V60_00970)	amiE	204491..205474 (+)	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	Regulator
G5V60_RS00975 (G5V60_00975)	dppF	205471..206475 (+)	1005	WP_001525880.1	dipeptide ABC transporter ATP-binding subunit DppF	-
G5V60_RS00980 (G5V60_00980)	yhjV	206505..207776 (-)	1272	WP_001525879.1	aromatic amino acid transport family protein	-
G5V60_RS00985 (G5V60_00985)	-	208252..208359 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
G5V60_RS00990 (G5V60_00990)	-	208735..208842 (+)	108	WP_000170748.1	type I toxin-antitoxin system toxin Ldr family protein	-
G5V60_RS00995 (G5V60_00995)	-	209218..209325 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35801.38 Da
• Isoelectric Point: 6.5813

>NTDB_id=372373 G5V60_RS00970 WP_001196477.1 204491..205474(+) (amiE) [Escherichia coli strain EC28]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=372373 G5V60_RS00970 WP_001196477.1 204491..205474(+) (amiE) [Escherichia coli strain EC28]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGTGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCTGGTAAATCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCAATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAAGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCTGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATTATCGTGATGTATGCAGGCCAGGTAGTAGAAACGGGCGATGCGCACGCT
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTACGCCA
CTGACAAATGCCGTGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• ABC transporter (ABC_tran) (24-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

3D structure

• AlphaFold DB: A0A0H2VBW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404