Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   THERJR_RS10355 Genome accession   NC_014152
Coordinates   2123960..2124874 (-) Length   304 a.a.
NCBI ID   WP_013120910.1    Uniprot ID   D5X8N0
Organism   Thermincola potens JR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2118960..2129874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THERJR_RS10335 (TherJR_2057) - 2120220..2120777 (-) 558 WP_013120906.1 aldolase -
  THERJR_RS10340 (TherJR_2058) - 2120799..2122058 (-) 1260 WP_013120907.1 adenosylhomocysteinase -
  THERJR_RS10345 (TherJR_2059) mtnA 2122059..2123099 (-) 1041 WP_013120908.1 S-methyl-5-thioribose-1-phosphate isomerase -
  THERJR_RS10350 (TherJR_2060) mtnP 2123149..2123943 (-) 795 WP_013120909.1 S-methyl-5'-thioadenosine phosphorylase -
  THERJR_RS10355 (TherJR_2061) pilA 2123960..2124874 (-) 915 WP_013120910.1 signal recognition particle-docking protein FtsY Machinery gene
  THERJR_RS10360 (TherJR_2062) - 2124945..2126087 (-) 1143 WP_013120911.1 hypothetical protein -
  THERJR_RS10365 (TherJR_2063) smc 2126170..2129739 (-) 3570 WP_013120912.1 chromosome segregation protein SMC -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 33564.97 Da        Isoelectric Point: 5.2258

>NTDB_id=37235 THERJR_RS10355 WP_013120910.1 2123960..2124874(-) (pilA) [Thermincola potens JR]
MGFFSKLKEKLTKTREGFVEKIESLVTGRKKIDEELYEELEEALIQSDVGINTALELVEELRRRVKERKVEDAAELKNIL
QEIITEVLMDDESRLELEKGKPNVIMVVGVNGVGKTTTIGKLAYQIKVGGRKVLLAAGDTFRAAAIDQLEIWSNRVGCDI
IKHKENADPAAVVFDAIQAARARNIDVLIIDTAGRLHTKSNLMEELKKVYRVINREMPDAPHEVLLVLDATTGQNAISQA
KLFGEAVGVTGIVLTKLDGTAKGGVIIGIKKELDVPVKLIGVGEQIEDLRPFKPGEFVEALFEK

Nucleotide


Download         Length: 915 bp        

>NTDB_id=37235 THERJR_RS10355 WP_013120910.1 2123960..2124874(-) (pilA) [Thermincola potens JR]
GTGGGTTTTTTTTCAAAACTAAAAGAAAAGCTGACTAAAACCCGCGAGGGTTTTGTAGAAAAAATCGAATCTCTGGTTAC
CGGCCGGAAAAAAATCGACGAAGAACTGTATGAAGAGTTGGAAGAAGCGCTGATCCAGAGTGATGTGGGAATTAATACCG
CCCTGGAACTGGTTGAAGAGCTGCGCAGAAGGGTCAAGGAGCGCAAGGTGGAAGACGCTGCCGAACTAAAAAATATCTTG
CAGGAAATCATAACTGAAGTACTTATGGATGACGAAAGCCGCTTGGAATTGGAAAAAGGTAAGCCCAATGTTATCATGGT
AGTGGGGGTAAACGGTGTCGGCAAAACAACCACTATCGGCAAACTGGCGTACCAGATCAAGGTCGGCGGCAGGAAAGTAT
TGCTGGCGGCCGGTGATACTTTCAGGGCTGCCGCTATTGACCAATTAGAAATATGGAGCAACAGGGTCGGCTGCGATATA
ATTAAGCATAAAGAAAATGCCGACCCGGCAGCGGTAGTTTTTGATGCTATTCAGGCTGCCCGGGCTAGGAATATAGATGT
ATTGATTATTGATACAGCGGGCAGGTTGCATACGAAGTCAAACCTGATGGAAGAATTGAAAAAGGTATACCGTGTTATTA
ACCGTGAAATGCCCGATGCTCCCCATGAAGTGCTGCTGGTTTTGGATGCTACCACCGGACAGAATGCCATCAGCCAGGCA
AAACTATTCGGCGAAGCCGTAGGGGTTACCGGCATAGTATTGACCAAACTCGACGGCACCGCCAAAGGCGGCGTTATCAT
AGGTATTAAAAAGGAATTGGATGTCCCTGTTAAACTGATAGGCGTCGGTGAACAGATAGAAGACCTCAGGCCTTTTAAAC
CCGGTGAATTTGTTGAGGCATTATTCGAAAAATAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(19-82)

SRP54-type protein, GTPase domain (SRP54)

(102-302)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D5X8N0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.026

100

0.48

Multiple sequence alignment