Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G6P91_RS20430 Genome accession   NZ_CP049085
Coordinates   4149130..4149570 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain p4A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4144130..4154570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6P91_RS20410 (G6P91_20425) aroP 4145130..4146500 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  G6P91_RS20415 (G6P91_20430) ampE 4146543..4147397 (-) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  G6P91_RS20420 (G6P91_20435) ampD 4147394..4147945 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G6P91_RS20425 (G6P91_20440) nadC 4148033..4148926 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G6P91_RS20430 (G6P91_20445) pilA 4149130..4149570 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  G6P91_RS20435 (G6P91_20450) gspE 4149580..4150965 (+) 1386 Protein_4069 type II secretion system protein GspE -
  G6P91_RS20440 (G6P91_20455) hofC 4150955..4152157 (+) 1203 WP_000157289.1 protein transport protein HofC -
  G6P91_RS20445 (G6P91_20460) guaC 4152192..4153235 (-) 1044 WP_001217320.1 GMP reductase -
  G6P91_RS20450 (G6P91_25785) - 4153391..4153435 (-) 45 WP_120795372.1 protein YacM -
  G6P91_RS20455 (G6P91_20465) coaE 4153460..4154080 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=372184 G6P91_RS20430 WP_000360914.1 4149130..4149570(+) (pilA) [Escherichia coli strain p4A]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=372184 G6P91_RS20430 WP_000360914.1 4149130..4149570(+) (pilA) [Escherichia coli strain p4A]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363