Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G6P89_RS20725 Genome accession   NZ_CP049077
Coordinates   4185258..4185698 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain p11A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4180258..4190698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6P89_RS20705 (G6P89_20705) aroP 4181258..4182628 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  G6P89_RS20710 (G6P89_20710) ampE 4182671..4183525 (-) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  G6P89_RS20715 (G6P89_20715) ampD 4183522..4184073 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G6P89_RS20720 (G6P89_20720) nadC 4184161..4185054 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G6P89_RS20725 (G6P89_20725) pilA 4185258..4185698 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  G6P89_RS20730 (G6P89_20730) gspE 4185708..4187093 (+) 1386 Protein_4124 type II secretion system protein GspE -
  G6P89_RS20735 (G6P89_20735) hofC 4187083..4188285 (+) 1203 WP_000157289.1 protein transport protein HofC -
  G6P89_RS20740 (G6P89_20740) guaC 4188320..4189363 (-) 1044 WP_001217320.1 GMP reductase -
  G6P89_RS20745 (G6P89_27600) - 4189519..4189563 (-) 45 WP_120795372.1 protein YacM -
  G6P89_RS20750 (G6P89_20745) coaE 4189588..4190208 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=372022 G6P89_RS20725 WP_000360914.1 4185258..4185698(+) (pilA) [Escherichia coli strain p11A]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=372022 G6P89_RS20725 WP_000360914.1 4185258..4185698(+) (pilA) [Escherichia coli strain p11A]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363