Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DXK01_RS08520 Genome accession   NZ_CP041379
Coordinates   2335547..2336914 (-) Length   455 a.a.
NCBI ID   WP_007667501.1    Uniprot ID   A0A3E4IJR8
Organism   Bacteroides intestinalis strain APC919/174     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2330547..2341914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXK01_RS08500 (DXK01_008500) - 2330783..2331364 (-) 582 WP_115502021.1 RNA polymerase sigma-70 factor -
  DXK01_RS08505 (DXK01_008505) - 2331655..2333280 (-) 1626 WP_115502020.1 NAD(P)/FAD-dependent oxidoreductase -
  DXK01_RS08510 (DXK01_008510) - 2333277..2333963 (-) 687 WP_007667505.1 hypothetical protein -
  DXK01_RS08515 (DXK01_008515) - 2333960..2335516 (-) 1557 WP_115502019.1 hypothetical protein -
  DXK01_RS08520 (DXK01_008520) radA 2335547..2336914 (-) 1368 WP_007667501.1 DNA repair protein RadA Machinery gene
  DXK01_RS08525 (DXK01_008525) - 2337207..2338247 (-) 1041 WP_044155428.1 asparaginase -
  DXK01_RS08535 (DXK01_008535) thrA 2338698..2341130 (+) 2433 WP_007667497.1 bifunctional aspartate kinase/homoserine dehydrogenase I -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50236.99 Da        Isoelectric Point: 6.4241

>NTDB_id=371735 DXK01_RS08520 WP_007667501.1 2335547..2336914(-) (radA) [Bacteroides intestinalis strain APC919/174]
MAKEKTVYVCNNCGQESPKWVGKCPSCGEWNTYVEEVIRKEVVNKRPVSGIETEKPKPIVLSEIEADDEPRIDLHDEELN
RVLGGGLVPGSLVLIGGEPGIGKSTLVLQTVLHIPKKRVLYVSGEESARQLKLRADRLSHNSSDCLIVCETSLEQIFVHI
KNTRPELVIIDSIQTISTESIESSPGSIAQVRECSASILRFAKETHTPVLLIGHINKEGSIAGPKVLEHIVDTVLQFEGD
QHYMYRILRSIKNRFGSTAELGIYEMRQDGLRQVSNPSELLLSQDHEGMSGIAIASAIEGIRPFLIETQALVSSAVYGNP
QRSATGFDIRRMNMLLAVLEKRVGFKLAQKDVFLNIAGGLKVNDPAIDLAVISAILSSNMDTAIEPEVCMAGEIGLSGEI
RPVNRIEQRIGEAEKLGFRRFVLPKYNLQGIDTKKLKIELIPVRKVEEAFRALFG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=371735 DXK01_RS08520 WP_007667501.1 2335547..2336914(-) (radA) [Bacteroides intestinalis strain APC919/174]
ATGGCAAAAGAAAAAACGGTTTATGTGTGCAATAACTGCGGACAAGAGTCTCCGAAATGGGTAGGCAAATGTCCGTCGTG
CGGAGAATGGAACACATATGTGGAAGAAGTGATACGAAAGGAAGTCGTCAACAAACGCCCGGTCTCCGGAATAGAAACAG
AAAAACCAAAACCTATTGTTCTTAGTGAAATAGAGGCGGATGACGAACCACGTATCGACCTGCACGATGAAGAACTGAAC
CGGGTATTGGGAGGTGGATTGGTTCCGGGCTCCCTAGTACTGATTGGCGGTGAACCGGGAATCGGTAAATCAACTTTGGT
GCTACAAACTGTGCTCCACATACCCAAAAAGCGCGTTCTCTATGTATCGGGTGAAGAAAGTGCCCGCCAATTAAAATTGC
GCGCCGACCGGTTATCGCACAATTCCAGCGATTGCCTTATTGTCTGCGAAACATCATTAGAGCAGATATTCGTACATATC
AAGAACACCCGTCCGGAACTGGTAATCATTGACTCTATACAGACCATCTCTACAGAGAGCATTGAATCATCGCCGGGAAG
CATTGCACAAGTACGCGAATGCTCAGCAAGCATCCTGCGCTTTGCCAAAGAAACACATACACCGGTATTGCTCATCGGAC
ATATTAATAAGGAAGGCAGCATTGCCGGACCAAAAGTCTTGGAACATATCGTAGACACGGTGTTGCAGTTTGAAGGTGAT
CAGCATTATATGTATCGCATCCTGCGTAGTATCAAGAATCGTTTTGGCAGCACAGCCGAACTGGGTATTTATGAAATGCG
ACAGGATGGTTTACGCCAAGTCAGCAATCCATCGGAGTTATTGTTAAGCCAGGATCACGAAGGTATGAGCGGCATAGCCA
TAGCTTCAGCCATAGAGGGGATACGTCCGTTCCTGATTGAAACTCAAGCATTAGTGAGCAGTGCGGTATATGGTAATCCG
CAACGTTCGGCCACGGGATTCGATATCCGAAGAATGAATATGCTATTGGCAGTACTGGAAAAACGGGTAGGCTTCAAACT
GGCTCAGAAGGATGTGTTCCTTAATATTGCCGGAGGATTGAAAGTTAATGATCCGGCGATAGACTTGGCTGTCATCAGTG
CCATACTGTCCAGCAATATGGATACGGCTATCGAACCGGAAGTCTGTATGGCAGGGGAAATAGGTTTGTCCGGCGAAATC
CGTCCGGTAAACCGAATTGAGCAAAGAATCGGCGAGGCGGAGAAGCTGGGATTCAGACGGTTTGTACTGCCGAAATACAA
TTTACAAGGAATTGATACGAAGAAACTCAAGATAGAACTAATACCGGTAAGAAAGGTAGAGGAAGCATTCAGAGCACTAT
TCGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(341-427)

AAA domain (AAA_25)

(71-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E4IJR8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

52.391

100

0.53
  radA Streptococcus mitis SK321

49.451

100

0.495
  radA Streptococcus mitis NCTC 12261

49.451

100

0.495
  radA Streptococcus pneumoniae Rx1

49.451

100

0.495
  radA Streptococcus pneumoniae D39

49.451

100

0.495
  radA Streptococcus pneumoniae R6

49.451

100

0.495
  radA Streptococcus pneumoniae TIGR4

49.451

100

0.495

Multiple sequence alignment