Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EXU85_RS23715 Genome accession   NZ_CP041360
Coordinates   5751846..5753231 (-) Length   461 a.a.
NCBI ID   WP_142774464.1    Uniprot ID   A0A515A3Z5
Organism   Spirosoma sp. KCTC 42546     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5746846..5758231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXU85_RS23685 (EXU85_23685) - 5747646..5748545 (-) 900 WP_142774458.1 sugar phosphate isomerase/epimerase -
  EXU85_RS23690 (EXU85_23690) - 5748681..5749556 (+) 876 WP_142774459.1 AraC family transcriptional regulator -
  EXU85_RS23695 (EXU85_23695) - 5749685..5750563 (-) 879 WP_142774460.1 alpha/beta fold hydrolase -
  EXU85_RS23700 (EXU85_23700) - 5750684..5751169 (+) 486 WP_142774461.1 DinB family protein -
  EXU85_RS23705 (EXU85_23705) - 5751190..5751480 (-) 291 WP_142774462.1 type II toxin-antitoxin system RelE/ParE family toxin -
  EXU85_RS23710 (EXU85_23710) - 5751597..5751764 (-) 168 WP_168207855.1 hypothetical protein -
  EXU85_RS23715 (EXU85_23715) radA 5751846..5753231 (-) 1386 WP_142774464.1 DNA repair protein RadA Machinery gene
  EXU85_RS23720 (EXU85_23720) - 5753471..5754142 (+) 672 WP_142774465.1 porin family protein -
  EXU85_RS23725 (EXU85_23725) - 5754275..5754694 (-) 420 WP_142774466.1 LytTR family DNA-binding domain-containing protein -
  EXU85_RS23730 (EXU85_23730) - 5754948..5755379 (-) 432 WP_142774467.1 LytTR family DNA-binding domain-containing protein -
  EXU85_RS23735 (EXU85_23735) pheS 5755748..5756791 (+) 1044 WP_142774468.1 phenylalanine--tRNA ligase subunit alpha -
  EXU85_RS23740 (EXU85_23740) - 5756892..5758091 (+) 1200 WP_142774469.1 exodeoxyribonuclease VII large subunit -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50239.75 Da        Isoelectric Point: 6.3205

>NTDB_id=371543 EXU85_RS23715 WP_142774464.1 5751846..5753231(-) (radA) [Spirosoma sp. KCTC 42546]
MAKLKTTYFCQSCGHQSAKWMGRCPVCGEWNTIVEELVQKDEPEKGGWRSPSSGPGGLKIAAKPKAIHAINYEEQPRVRT
TDLELNRVLGGGIVAGSLVLIGGEPGIGKSTLLLQIALSLAGMRVLYVSGEESEQQIKMRAERLDAPTSDCHVMTETSTQ
NIFRVVEHFEPEVLIIDSIQTMQSSLVESGAGSVSQVRECTAEFMKYAKESGVPVFMIGHITKEGSLAGPKVLEHMVDTV
LTFEGDRHTTYRILRTTKNRFGSTDELGIYEMLGSGLRQVTNPSEILISQRDEALSGVTIGSMLEGNRPLMIETQALVSV
ANYGTPQRSSTGFDAKRLNMLLAVLEKRGGFRLGQQDVFLNIAGGLRVEDPAIDLAVCAAIVSSYEDIAIPPSVAFAAEV
GLGGEVRAVSRIESRIAEAEKLGFKQMFISKYNMKGLDTKGYKIHIRPVSRLDEVFQGVLI

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=371543 EXU85_RS23715 WP_142774464.1 5751846..5753231(-) (radA) [Spirosoma sp. KCTC 42546]
ATGGCTAAATTAAAGACTACTTATTTCTGTCAGAGTTGCGGGCATCAGTCTGCTAAATGGATGGGTCGCTGCCCAGTCTG
TGGTGAGTGGAATACAATTGTGGAGGAGCTCGTTCAGAAAGATGAACCCGAGAAAGGCGGCTGGCGAAGTCCCTCATCTG
GCCCAGGCGGACTTAAAATTGCAGCTAAGCCGAAAGCAATCCATGCCATTAATTATGAAGAACAGCCGCGTGTTCGGACG
ACTGATCTTGAACTGAACCGTGTACTCGGTGGAGGTATTGTGGCAGGCTCACTGGTGCTCATTGGTGGAGAACCTGGTAT
CGGGAAATCTACACTGTTACTTCAAATTGCGCTCAGCTTAGCGGGCATGCGTGTACTTTACGTGTCGGGTGAGGAGAGTG
AGCAGCAGATAAAAATGCGGGCCGAACGCCTGGATGCACCTACCAGCGATTGCCATGTGATGACGGAAACCTCTACCCAA
AATATCTTTCGGGTGGTAGAGCATTTTGAGCCCGAAGTCCTGATTATTGACTCGATTCAGACCATGCAATCGTCATTGGT
GGAATCGGGGGCAGGCAGTGTCTCGCAGGTTCGTGAATGCACGGCTGAGTTTATGAAATATGCCAAAGAATCAGGTGTGC
CGGTGTTCATGATTGGCCATATTACCAAAGAGGGGTCCCTGGCCGGCCCGAAAGTGCTGGAACACATGGTCGATACTGTA
CTGACTTTCGAGGGCGACCGACACACCACATACCGAATTCTACGAACCACTAAAAACCGTTTTGGAAGCACCGACGAATT
AGGTATTTATGAAATGCTGGGTAGTGGCCTACGCCAAGTTACTAACCCATCCGAAATTCTGATTTCGCAACGTGATGAAG
CACTAAGTGGCGTCACGATTGGCTCAATGCTGGAAGGGAATCGCCCTCTCATGATTGAAACCCAGGCCCTTGTAAGCGTG
GCCAACTATGGCACACCGCAACGTAGTAGTACAGGCTTTGATGCTAAACGCTTGAATATGCTGCTGGCTGTCCTGGAAAA
ACGAGGTGGGTTTCGGCTTGGTCAGCAGGACGTTTTCCTGAACATAGCAGGTGGTTTACGTGTGGAAGACCCGGCCATTG
ATCTGGCCGTTTGCGCGGCTATTGTATCGAGTTACGAAGACATTGCCATTCCGCCATCGGTGGCCTTTGCGGCTGAAGTT
GGTCTTGGGGGCGAAGTGCGAGCAGTGAGCAGGATAGAATCCCGCATTGCTGAAGCCGAAAAACTGGGCTTTAAACAGAT
GTTTATTTCCAAATACAACATGAAGGGCCTGGATACCAAGGGATACAAAATTCATATCAGGCCTGTATCGAGACTAGATG
AGGTATTTCAGGGGGTTTTGATTTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(84-225)

Subunit ChlI of Mg-chelatase (ChlI)

(347-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A515A3Z5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.515

100

0.516
  radA Streptococcus mitis NCTC 12261

48.807

100

0.488
  radA Streptococcus mitis SK321

48.807

100

0.488
  radA Streptococcus pneumoniae Rx1

48.807

100

0.488
  radA Streptococcus pneumoniae D39

48.807

100

0.488
  radA Streptococcus pneumoniae R6

48.807

100

0.488
  radA Streptococcus pneumoniae TIGR4

48.807

100

0.488

Multiple sequence alignment