Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FLP15_RS00950 Genome accession   NZ_CP041356
Coordinates   210019..211380 (-) Length   453 a.a.
NCBI ID   WP_142765663.1    Uniprot ID   A0A514Z620
Organism   Lactococcus protaetiae strain KACC 19320     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 205019..216380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLP15_RS00940 (FLP15_00940) - 205244..205570 (-) 327 WP_120772480.1 LPXTG cell wall anchor domain-containing protein -
  FLP15_RS00945 (FLP15_00945) - 205585..209709 (-) 4125 WP_142765662.1 hypothetical protein -
  FLP15_RS00950 (FLP15_00950) radA 210019..211380 (-) 1362 WP_142765663.1 DNA repair protein RadA Machinery gene
  FLP15_RS00960 (FLP15_00960) - 211936..212208 (-) 273 WP_223804671.1 hypothetical protein -
  FLP15_RS00965 (FLP15_00965) - 212326..213246 (-) 921 WP_142765665.1 YafY family protein -
  FLP15_RS00970 (FLP15_00970) - 213336..213818 (+) 483 WP_142765666.1 AraC family transcriptional regulator -
  FLP15_RS00975 (FLP15_00975) rplQ 214221..214601 (-) 381 WP_142765667.1 50S ribosomal protein L17 -
  FLP15_RS00980 (FLP15_00980) - 214617..215555 (-) 939 WP_142765668.1 DNA-directed RNA polymerase subunit alpha -
  FLP15_RS00985 (FLP15_00985) rpsK 215606..215989 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  FLP15_RS00990 (FLP15_00990) rpsM 216010..216375 (-) 366 WP_142765669.1 30S ribosomal protein S13 -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49981.33 Da        Isoelectric Point: 5.0005

>NTDB_id=371475 FLP15_RS00950 WP_142765663.1 210019..211380(-) (radA) [Lactococcus protaetiae strain KACC 19320]
MAKKKSSFICQNCGYKSATYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPMKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVMYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRSEIE
HLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLIEVNNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGLLMQNQDAYLKSAGGVKLDEPAIDLAVAIAVASSYKELSTDARECFIGEIGLTGEIR
RVTRIEQRINEAAKLGFKKVYAPKNSISGIEIPENIEVVGVTTLMECMKLVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=371475 FLP15_RS00950 WP_142765663.1 210019..211380(-) (radA) [Lactococcus protaetiae strain KACC 19320]
ATGGCTAAGAAAAAATCATCCTTTATTTGCCAAAATTGTGGCTATAAATCGGCAACATATCTTGGACGGTGTCCAAACTG
TGGAGAATGGTCTTCATTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAAAGAGTTTCAATGACTGGTGAACGTT
CAAAACCGATGAAACTAGATGAAGTTGAACTTTTTGACACACCAAGAATCGAGACAGACTTAGACGAGTTTAATCGCGTA
CTTGGTGGAGGTGTTGTACCAGGTTCTTTAGTCTTAATCGGTGGAGACCCAGGGATTGGTAAATCAACATTGCTTCTACA
AGTATCCACACAGTTGGCATCACGTGGCCGTGTCATGTATGTCAGTGGTGAAGAGTCTGCTCAGCAGATTAAATTACGAG
CAGAGCGACTTGGAGATATTGATAGAGATTTTTATCTTTATGCTGAAACAAATATGCAGTCTATTCGCTCAGAAATAGAA
CATTTGAAACCCAATTTTCTGATTATTGACTCTATCCAGACGATTATGACACCAGAGATACAGTCTACACAAGGGTCAGT
AAGTCAAGTACGTGAAGTGACAGGAGAGCTGATGCAGATTGCTAAAACCAATGATATTGCAACTTTCATCGTTGGTCACG
TCACAAAAGAAGGACAACTTGCAGGCCCTCGGATGCTTGAGCATATGGTAGATACAGTTTTATATTTTGAAGGTGAAAGA
AATAACACTTTTAGGATTTTGCGCGCAGTAAAAAACCGTTTTGGCTCAACCAATGAAATAGGGATTTTTGAGATGCAAGG
CCATGGTTTGATTGAGGTCAATAATCCTTCGGAGGTTTTTCTAGAAGAACGCTTAGAAGGCTCAACTGGCTCTGCGATTG
TTTGTGCGCTTGAGGGGACACGACCTATCTTGGTAGAAATTCAAGCCTTGACCACACCGACGATGTTTGGTAATGCCAAA
CGGACGACATCTGGACTTGATTTTAACCGCGTAAGTCTGATTATGGCAGTTTTAGAAAAACGTGCAGGTTTGCTCATGCA
AAATCAAGATGCTTATCTTAAATCTGCTGGAGGTGTCAAATTAGATGAGCCTGCTATTGACCTTGCGGTAGCTATCGCAG
TAGCTTCAAGTTATAAAGAACTATCGACAGATGCGCGTGAATGTTTTATCGGCGAAATTGGTCTGACTGGTGAAATTCGT
AGGGTTACTCGGATTGAGCAACGAATTAATGAAGCAGCAAAGCTCGGTTTTAAAAAAGTTTACGCCCCTAAAAACTCAAT
ATCAGGAATAGAAATCCCTGAAAATATCGAAGTAGTTGGTGTGACAACACTAATGGAGTGCATGAAATTAGTCTTCTCCT
GA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(70-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A514Z620

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

79.249

100

0.792
  radA Streptococcus pneumoniae D39

79.249

100

0.792
  radA Streptococcus pneumoniae R6

79.249

100

0.792
  radA Streptococcus pneumoniae TIGR4

79.249

100

0.792
  radA Streptococcus mitis NCTC 12261

79.249

100

0.792
  radA Streptococcus mitis SK321

79.249

100

0.792
  radA Bacillus subtilis subsp. subtilis str. 168

61.81

100

0.618

Multiple sequence alignment