Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FKL89_RS18225 Genome accession   NZ_CP041352
Coordinates   4023050..4024072 (-) Length   340 a.a.
NCBI ID   WP_156864147.1    Uniprot ID   -
Organism   Casimicrobium huifangae strain SJ-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4018050..4029072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKL89_RS18200 - 4018687..4019376 (-) 690 WP_156864142.1 hypothetical protein -
  FKL89_RS18205 - 4019466..4020146 (-) 681 WP_156864143.1 hypothetical protein -
  FKL89_RS18210 - 4020304..4020840 (+) 537 WP_156864144.1 glutathione peroxidase -
  FKL89_RS18215 - 4020968..4022047 (+) 1080 WP_156864145.1 ABC transporter ATP-binding protein -
  FKL89_RS18220 - 4022200..4023024 (+) 825 WP_156864146.1 glycosyltransferase family 2 protein -
  FKL89_RS18225 pilA 4023050..4024072 (-) 1023 WP_156864147.1 signal recognition particle-docking protein FtsY Machinery gene
  FKL89_RS18230 rsmD 4024182..4024778 (+) 597 WP_156864148.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  FKL89_RS18235 coaD 4024799..4025296 (+) 498 WP_156864149.1 pantetheine-phosphate adenylyltransferase -
  FKL89_RS18240 - 4025601..4027811 (+) 2211 WP_156864150.1 carbohydrate binding family 9 domain-containing protein -
  FKL89_RS18245 - 4027886..4028152 (+) 267 WP_156864151.1 YfhL family 4Fe-4S dicluster ferredoxin -
  FKL89_RS18250 - 4028149..4028553 (+) 405 WP_156864152.1 GlcG/HbpS family heme-binding protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 35784.56 Da        Isoelectric Point: 6.2803

>NTDB_id=371411 FKL89_RS18225 WP_156864147.1 4023050..4024072(-) (pilA) [Casimicrobium huifangae strain SJ-1]
MWGLFKKKPQPDASVAPAPAAVDAPATTMPATESALAAVVAPVEKKGWLDKLKSGLTATRDKLGLKVLFAGKLDEDTLEQ
LEAQLLMADCGMAATQHLLDDLRKRWKLAGGQGDPRAMLVDALTDLLQPLEQPFAVARESPFVIMIAGVNGAGKTTSIGK
LAKYLQQQGASVLLAAGDTFRAAAREQLAIWGERNNVTVIQQAGGDPAAVMFDAVTAGKARGTDVVMCDTAGRLPTQLHL
MDELKKAKRVIGKAMDSAPHEVLLVLDGTTGQNALAQVKAFDDALGLSGLIMTKLDGSAKGGVICAIAKERPIALRFIGV
GEGIDDLQPFVAREFAEALV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=371411 FKL89_RS18225 WP_156864147.1 4023050..4024072(-) (pilA) [Casimicrobium huifangae strain SJ-1]
ATGTGGGGCTTGTTCAAGAAGAAACCGCAACCCGACGCCAGTGTTGCGCCAGCACCGGCAGCAGTTGACGCCCCGGCAAC
AACAATGCCAGCGACGGAATCGGCGCTGGCGGCAGTGGTTGCACCGGTCGAAAAGAAAGGCTGGCTGGACAAGCTCAAAT
CCGGCCTCACCGCGACGCGCGACAAGCTCGGCCTCAAGGTGCTGTTCGCCGGCAAGCTTGACGAAGACACACTGGAGCAA
CTCGAAGCCCAACTGCTGATGGCGGACTGTGGCATGGCGGCGACGCAGCATCTGCTCGACGATCTGCGCAAGCGCTGGAA
GCTGGCCGGCGGTCAGGGCGATCCGCGCGCCATGCTGGTGGATGCCTTGACCGATCTGCTGCAGCCGCTCGAGCAGCCGT
TCGCGGTGGCGAGAGAATCTCCCTTCGTCATCATGATCGCCGGCGTCAATGGTGCCGGCAAAACGACCAGCATCGGCAAG
CTTGCCAAATACCTGCAGCAGCAGGGTGCGAGCGTGCTGCTGGCAGCGGGTGACACCTTCCGCGCTGCGGCACGCGAGCA
GCTCGCGATCTGGGGCGAGCGCAACAACGTCACCGTGATCCAGCAGGCTGGTGGTGATCCGGCAGCGGTGATGTTCGACG
CCGTCACTGCCGGCAAGGCGCGCGGTACCGATGTGGTGATGTGCGACACGGCGGGCCGCCTGCCGACACAGCTGCACCTG
ATGGACGAGTTGAAGAAGGCGAAGCGGGTGATCGGCAAGGCGATGGACAGCGCGCCGCACGAAGTGTTGCTGGTGCTCGA
CGGCACCACTGGCCAGAACGCACTGGCTCAGGTCAAGGCCTTCGACGATGCACTTGGGCTCAGCGGCCTCATCATGACCA
AGCTTGATGGCAGCGCCAAGGGCGGCGTGATCTGCGCCATCGCCAAAGAGCGGCCAATTGCGCTGCGCTTCATCGGCGTT
GGTGAAGGCATCGACGATCTGCAGCCATTCGTGGCGCGCGAGTTTGCCGAGGCGCTGGTTTAG

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(141-339)

SRP54-type protein, helical bundle domain (SRP54_N)

(70-107)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

58.359

96.765

0.565

Multiple sequence alignment