Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FK538_RS02620 Genome accession   NZ_CP041295
Coordinates   545691..546488 (+) Length   265 a.a.
NCBI ID   WP_104470591.1    Uniprot ID   -
Organism   Acinetobacter indicus strain 80-1-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540691..551488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FK538_RS02590 (FK538_02595) - 541007..541426 (+) 420 WP_005182377.1 hypothetical protein -
  FK538_RS02595 (FK538_02600) - 541594..542877 (+) 1284 WP_104484270.1 glycosyltransferase family 4 protein -
  FK538_RS02600 (FK538_02605) - 542910..543461 (+) 552 WP_005182381.1 phosphatase PAP2 family protein -
  FK538_RS02605 (FK538_02610) iscX 543556..543756 (+) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  FK538_RS02610 (FK538_02615) ndk 543866..544297 (+) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  FK538_RS02615 (FK538_02620) rlmN 544420..545655 (+) 1236 WP_005182389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FK538_RS02620 (FK538_02625) pilF 545691..546488 (+) 798 WP_104470591.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FK538_RS02625 (FK538_02630) - 546479..547267 (+) 789 WP_045795990.1 helix-turn-helix domain-containing protein -
  FK538_RS02630 (FK538_02635) ispG 547290..548411 (+) 1122 WP_005182396.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FK538_RS02635 (FK538_02640) hisS 548415..549710 (+) 1296 WP_168370367.1 histidine--tRNA ligase -
  FK538_RS02640 (FK538_02645) - 549741..550445 (+) 705 WP_005182401.1 YfgM family protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29946.85 Da        Isoelectric Point: 8.3587

>NTDB_id=371038 FK538_RS02620 WP_104470591.1 545691..546488(+) (pilF) [Acinetobacter indicus strain 80-1-2]
MYKFTTISTLCVAAWMSTGCQTTTPTLSPKDPEKAIQVRTQLAAEYLKSGDLDSAKRALDQALETDSRDSMANMMMGVLL
QQEGSKLNLEKADTYFKRAISADPNNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDMA
NAEKSFKQALQANRDSYVSMLELAEIFYFNQQTPAASRMYEQFVRAVGQKNQGARALWIGIRVARANGDQLGQQVLVNQL
RALFPESQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=371038 FK538_RS02620 WP_104470591.1 545691..546488(+) (pilF) [Acinetobacter indicus strain 80-1-2]
ATGTATAAATTCACAACCATCAGCACACTGTGTGTGGCTGCATGGATGAGCACCGGCTGTCAAACCACAACTCCTACTTT
AAGTCCGAAAGATCCGGAAAAGGCAATTCAGGTGCGTACCCAGCTGGCGGCCGAGTATTTAAAATCGGGTGATTTAGACT
CGGCCAAACGTGCCCTGGATCAGGCACTGGAAACAGATTCGCGTGATTCCATGGCCAATATGATGATGGGCGTGCTGCTA
CAGCAAGAAGGCAGCAAGCTGAATCTGGAGAAGGCCGATACGTATTTTAAGCGTGCCATCTCTGCCGATCCGAACAATGC
GCAGGCGCGTAACAACTATGGTACGTATTTGTATCAGGTTGAACGCTATAATGATGCCATTGAACAGCTGAGTATTGCCG
GGGCAACACTCGGTTATGATCAACGTTTTCGTGCATTGGAAAACGTGGGGCGGATTTACCTGAAACTGGGCGATATGGCA
AATGCTGAAAAATCATTCAAGCAGGCCCTGCAGGCAAACCGTGACTCCTATGTTTCAATGTTAGAGTTGGCGGAAATTTT
TTATTTCAACCAGCAGACTCCTGCCGCATCCAGAATGTATGAACAGTTTGTGCGTGCTGTGGGACAAAAAAATCAAGGTG
CGCGTGCATTGTGGATTGGGATCCGTGTTGCACGAGCAAATGGCGATCAGTTGGGACAGCAGGTGCTGGTCAATCAGCTT
CGGGCATTGTTCCCTGAAAGCCAGGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(144-175)

Tetratricopeptide repeat (TPR_17)

(96-128)

Tetratricopeptide repeat (TPR_16)

(42-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.992

97.736

0.762
  pilF Acinetobacter baylyi ADP1

72.119

100

0.732

Multiple sequence alignment