Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FK537_RS05065 Genome accession   NZ_CP041291
Coordinates   970777..971574 (-) Length   265 a.a.
NCBI ID   WP_104470591.1    Uniprot ID   -
Organism   Acinetobacter indicus strain 94-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 965777..976574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FK537_RS05045 (FK537_05085) - 966820..967524 (-) 705 WP_075167363.1 YfgM family protein -
  FK537_RS05050 (FK537_05090) hisS 967555..968850 (-) 1296 WP_104505572.1 histidine--tRNA ligase -
  FK537_RS05055 (FK537_05095) ispG 968854..969975 (-) 1122 WP_160232075.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FK537_RS05060 (FK537_05100) - 969998..970786 (-) 789 WP_168369714.1 helix-turn-helix domain-containing protein -
  FK537_RS05065 (FK537_05105) pilF 970777..971574 (-) 798 WP_104470591.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FK537_RS05070 (FK537_05110) rlmN 971610..972845 (-) 1236 WP_005182389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FK537_RS05075 (FK537_05115) ndk 972968..973399 (-) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  FK537_RS05080 (FK537_05120) iscX 973509..973709 (-) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  FK537_RS05085 (FK537_05125) - 973804..974355 (-) 552 WP_005182381.1 phosphatase PAP2 family protein -
  FK537_RS05090 (FK537_05130) - 974388..975671 (-) 1284 WP_163142800.1 glycosyltransferase family 4 protein -
  FK537_RS05095 (FK537_05135) - 975839..976258 (-) 420 WP_005182377.1 hypothetical protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29946.85 Da        Isoelectric Point: 8.3587

>NTDB_id=370988 FK537_RS05065 WP_104470591.1 970777..971574(-) (pilF) [Acinetobacter indicus strain 94-2]
MYKFTTISTLCVAAWMSTGCQTTTPTLSPKDPEKAIQVRTQLAAEYLKSGDLDSAKRALDQALETDSRDSMANMMMGVLL
QQEGSKLNLEKADTYFKRAISADPNNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDMA
NAEKSFKQALQANRDSYVSMLELAEIFYFNQQTPAASRMYEQFVRAVGQKNQGARALWIGIRVARANGDQLGQQVLVNQL
RALFPESQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=370988 FK537_RS05065 WP_104470591.1 970777..971574(-) (pilF) [Acinetobacter indicus strain 94-2]
ATGTATAAATTCACAACCATCAGCACATTGTGTGTGGCTGCATGGATGAGCACCGGCTGTCAAACCACAACTCCTACTTT
AAGTCCGAAAGATCCGGAAAAGGCAATTCAGGTGCGTACCCAGCTGGCGGCCGAGTATTTAAAATCGGGTGATTTAGACT
CGGCCAAACGTGCCCTGGATCAGGCGCTGGAAACAGATTCGCGTGATTCCATGGCCAATATGATGATGGGCGTGCTGCTA
CAGCAAGAAGGCAGCAAGCTGAATCTGGAGAAGGCCGATACGTATTTTAAGCGTGCCATCTCTGCCGATCCGAACAATGC
GCAGGCGCGTAACAACTATGGTACGTATTTGTATCAGGTTGAACGCTATAATGATGCCATTGAACAGCTGAGTATTGCCG
GGGCAACACTCGGTTATGATCAACGTTTTCGTGCATTGGAAAACGTGGGGCGGATTTACCTGAAACTGGGCGATATGGCG
AATGCTGAAAAATCATTCAAGCAGGCCCTGCAGGCAAACCGTGACTCCTATGTTTCAATGTTAGAGTTGGCGGAAATTTT
TTATTTCAACCAGCAGACTCCTGCAGCATCCAGAATGTATGAACAGTTTGTGCGTGCTGTGGGACAAAAAAATCAAGGTG
CGCGTGCATTGTGGATTGGGATCCGTGTTGCACGAGCAAATGGCGATCAGTTGGGACAGCAGGTGCTAGTCAATCAGCTT
CGGGCATTGTTCCCTGAAAGCCAGGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(144-175)

Tetratricopeptide repeat (TPR_17)

(96-128)

Tetratricopeptide repeat (TPR_16)

(42-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.992

97.736

0.762
  pilF Acinetobacter baylyi ADP1

72.119

100

0.732

Multiple sequence alignment