Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FKX85_RS04850 Genome accession   NZ_CP041253
Coordinates   1168709..1170085 (+) Length   458 a.a.
NCBI ID   WP_141613655.1    Uniprot ID   A0A514CF11
Organism   Echinicola soli strain LN3S3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1163709..1175085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKX85_RS04835 (FKX85_04835) - 1164027..1164266 (+) 240 WP_141613653.1 GIY-YIG nuclease family protein -
  FKX85_RS04840 (FKX85_04840) - 1164450..1164689 (+) 240 WP_141613653.1 GIY-YIG nuclease family protein -
  FKX85_RS04845 (FKX85_04845) polA 1165713..1168529 (+) 2817 WP_141613654.1 DNA polymerase I -
  FKX85_RS04850 (FKX85_04850) radA 1168709..1170085 (+) 1377 WP_141613655.1 DNA repair protein RadA Machinery gene
  FKX85_RS04855 (FKX85_04855) - 1170202..1170624 (+) 423 WP_141613656.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  FKX85_RS04860 (FKX85_04860) - 1170705..1171361 (-) 657 WP_141613657.1 HAD family phosphatase -
  FKX85_RS04865 (FKX85_04865) - 1171518..1171901 (+) 384 WP_141613658.1 (deoxy)nucleoside triphosphate pyrophosphohydrolase -
  FKX85_RS04870 (FKX85_04870) - 1171901..1175038 (+) 3138 WP_141613659.1 DUF3427 domain-containing protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50305.65 Da        Isoelectric Point: 6.4214

>NTDB_id=370809 FKX85_RS04850 WP_141613655.1 1168709..1170085(+) (radA) [Echinicola soli strain LN3S3]
MPKIKTAYFCQNCGAQSPKWAGKCPACGEWNTYVEEVIHKEETGRGSWKQGAEKTKRSNSPRKLQEINYEEHPRLVTQDP
ELDRVLGGGIVPGSLTLIGGEPGIGKSTLMLQIALVLNQTKVLYVSGEESESQIKMRADRMQFHSDNCFVLSETNTQTIF
QQIETVKPEVLVIDSIQTLHSKHVESAAGSVSQVRECTAELMKFAKETGTPVFLIGHITKDGSIAGPKILEHMVDTVLQF
EGDRHLSYRILRTSKNRFGSTNELGIYEMRAEGLRGVANPSEILLSQREEVLNGVAIGAMLEGNRPLLIEIQSLISPATY
GTPQRSSTGHDAKRLNMLLAVLEKRGGMRLGQQDVFLNVAGGMRVDDPGLDLAVCAALLSSYEDTPVSPDLCFAGEVGLG
GEIRAVNRIENRIAEADKLGFKKIIVSKYAVKGVDLSTFGIEVIPVTKLDEMYQRLFS

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=370809 FKX85_RS04850 WP_141613655.1 1168709..1170085(+) (radA) [Echinicola soli strain LN3S3]
ATGCCAAAAATAAAGACGGCTTATTTCTGCCAAAACTGCGGTGCCCAAAGTCCTAAGTGGGCGGGCAAGTGTCCCGCCTG
TGGAGAGTGGAACACCTATGTGGAAGAGGTTATCCACAAAGAAGAAACAGGCCGTGGAAGCTGGAAACAGGGTGCTGAAA
AAACAAAACGCAGCAATTCGCCCAGAAAATTACAAGAGATCAACTACGAAGAGCATCCTCGTTTGGTCACGCAGGATCCT
GAATTGGACCGTGTTTTGGGAGGAGGAATCGTTCCAGGCTCCCTGACCCTGATAGGGGGTGAGCCGGGCATTGGCAAATC
CACCTTGATGCTGCAGATAGCACTGGTGCTGAACCAAACCAAAGTGCTGTATGTCTCCGGTGAAGAAAGCGAGAGCCAGA
TCAAGATGCGGGCGGACAGAATGCAGTTTCATTCAGACAACTGCTTCGTGCTTTCGGAGACCAATACTCAAACCATTTTC
CAGCAGATAGAAACGGTGAAACCTGAAGTGTTGGTCATCGACTCTATCCAAACCCTCCACAGCAAACACGTGGAGTCCGC
CGCAGGAAGTGTTTCACAAGTAAGGGAATGCACGGCAGAACTCATGAAATTTGCCAAAGAAACCGGTACTCCGGTTTTTC
TAATTGGCCATATCACCAAGGACGGCTCCATCGCCGGACCAAAGATCCTGGAGCATATGGTGGACACCGTGTTGCAATTT
GAGGGAGATCGACACCTGTCTTACCGGATACTGCGTACTTCAAAAAACCGTTTTGGCTCTACCAATGAGCTCGGCATCTA
TGAAATGCGTGCAGAAGGCCTCCGTGGCGTGGCCAATCCTTCAGAGATCCTCTTGAGCCAGCGAGAGGAAGTGCTCAATG
GCGTGGCCATCGGTGCCATGCTGGAGGGAAACCGCCCACTGCTGATCGAGATCCAATCGCTGATCAGTCCCGCCACCTAT
GGCACTCCGCAGCGAAGCAGTACCGGCCATGATGCCAAACGGCTGAACATGCTCTTGGCCGTATTGGAGAAACGCGGCGG
AATGCGCTTGGGACAGCAGGATGTTTTCTTGAATGTGGCTGGAGGGATGCGCGTGGATGATCCGGGCTTGGATTTGGCCG
TATGTGCTGCGTTGCTTTCTTCCTATGAAGATACACCGGTGTCACCAGACTTGTGCTTTGCGGGTGAAGTGGGGCTGGGC
GGTGAAATCCGCGCCGTCAACCGCATCGAAAACCGTATTGCCGAAGCAGATAAGCTAGGCTTCAAAAAGATCATCGTTTC
CAAATATGCCGTAAAAGGCGTTGACTTATCCACATTTGGCATTGAAGTAATCCCTGTCACCAAGCTGGATGAGATGTATC
AGCGTTTGTTCAGCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(75-222)

Subunit ChlI of Mg-chelatase (ChlI)

(346-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A514CF11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

53.863

98.908

0.533
  radA Streptococcus pneumoniae Rx1

50.87

100

0.511
  radA Streptococcus pneumoniae D39

50.87

100

0.511
  radA Streptococcus pneumoniae R6

50.87

100

0.511
  radA Streptococcus pneumoniae TIGR4

50.87

100

0.511
  radA Streptococcus mitis SK321

50.652

100

0.509
  radA Streptococcus mitis NCTC 12261

50.652

100

0.509

Multiple sequence alignment