Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Electrica_RS02405 Genome accession   NZ_CP041247
Coordinates   502776..504209 (-) Length   477 a.a.
NCBI ID   WP_131048464.1    Uniprot ID   A0AAJ5QVE0
Organism   Klebsiella electrica strain DSM 102253     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 497776..509209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Electrica_RS02375 (electrica_00475) elbB 499299..499952 (+) 654 WP_131048462.1 isoprenoid biosynthesis glyoxalase ElbB -
  Electrica_RS02380 (electrica_00476) mtgA 499949..500674 (+) 726 WP_131048463.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  Electrica_RS02385 (electrica_00477) npr 500736..501008 (-) 273 WP_004868176.1 PTS phosphocarrier protein NPr -
  Electrica_RS02390 (electrica_00478) rapZ 501005..501859 (-) 855 WP_004868172.1 RNase adapter RapZ -
  Electrica_RS02395 (electrica_00479) ptsN 501905..502393 (-) 489 WP_015585655.1 PTS IIA-like nitrogen regulatory protein PtsN -
  Electrica_RS02400 (electrica_00480) hpf 502466..502753 (-) 288 WP_004868168.1 ribosome hibernation promoting factor -
  Electrica_RS02405 (electrica_00481) rpoN/rpoN1 502776..504209 (-) 1434 WP_131048464.1 RNA polymerase factor sigma-54 Machinery gene
  Electrica_RS02410 (electrica_00482) lptB 504257..504982 (-) 726 WP_004206203.1 LPS export ABC transporter ATP-binding protein -
  Electrica_RS02415 (electrica_00483) lptA 504989..505534 (-) 546 WP_100684859.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  Electrica_RS02420 (electrica_00484) lptC 505503..506078 (-) 576 WP_100684858.1 LPS export ABC transporter periplasmic protein LptC -
  Electrica_RS02425 (electrica_00485) kdsC 506075..506641 (-) 567 WP_131048465.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  Electrica_RS02430 (electrica_00486) kdsD 506656..507660 (-) 1005 WP_407081244.1 arabinose-5-phosphate isomerase KdsD -
  Electrica_RS02435 (electrica_00487) - 507657..508634 (-) 978 WP_100684855.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54017.96 Da        Isoelectric Point: 4.3873

>NTDB_id=370787 Electrica_RS02405 WP_131048464.1 502776..504209(-) (rpoN/rpoN1) [Klebsiella electrica strain DSM 102253]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHDEVETKEVEDRESLDTVDALEQKDMPE
ELPLDASWDEIYTAGTPSGNGVDYQDDELPVYQGETTQSLQDYLMWQVELTPFTDTDRAIATSIVDAVDDTGYLTIRLED
IVESIDDDEIGLEEVEAVLKRIQRFDPVGVAAKDLRDCLLVQLSQYAKETPWLEEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKVGGRWVVELNSDSIPRLKINQHYAAMGNSARNDADSQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQSFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=370787 Electrica_RS02405 WP_131048464.1 502776..504209(-) (rpoN/rpoN1) [Klebsiella electrica strain DSM 102253]
ATGAAGCAAGGTTTGCAATTAAGGCTAAGCCAACAGCTTGCCATGACGCCACAACTGCAACAGGCTATTCGCTTGCTGCA
GCTGTCCACGTTAGAACTCCAGCAAGAACTCCAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAACCGATCTTCACG
ATGAGGTAGAAACCAAAGAGGTTGAGGACCGCGAATCGCTGGACACCGTCGACGCTCTTGAACAAAAAGATATGCCGGAA
GAGCTGCCGCTCGACGCCAGCTGGGATGAAATCTACACCGCCGGCACGCCGTCCGGGAACGGCGTTGATTACCAGGACGA
CGAGCTGCCTGTTTATCAAGGCGAAACGACCCAAAGCCTGCAGGATTACCTGATGTGGCAGGTTGAACTGACGCCGTTTA
CCGACACCGATCGCGCGATTGCCACATCGATTGTCGATGCCGTCGATGACACCGGCTACCTCACCATTCGGCTGGAAGAT
ATTGTCGAGAGTATCGACGATGACGAAATTGGTCTCGAAGAAGTTGAAGCGGTACTTAAGCGCATCCAGCGCTTTGACCC
GGTCGGCGTCGCGGCAAAAGATCTGCGCGACTGTCTGCTGGTTCAGCTTTCACAATATGCCAAAGAGACGCCGTGGCTGG
AAGAAGCCCGGCTTATCATCAGCGATCATCTCGATCTGCTCGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACCCGA
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTTAATCTGATCCAGTCGCTCGATCCGCGCCCCGGTCAGTCGATCCAGACCGG
CGAGCCGGAATACGTCATTCCCGACGTACTGGTACGTAAGGTCGGCGGCCGCTGGGTCGTGGAGCTTAACTCCGACAGCA
TCCCACGCCTGAAGATCAACCAGCATTATGCGGCCATGGGCAACAGCGCGCGCAATGATGCAGACAGCCAGTTTATCCGC
AGCAACCTGCAGGAGGCTCGCTGGCTAATCAAGAGTCTGGAAAGCCGCAATGATACGCTGCTGCGCGTTAGCCGCTGCAT
CGTTGAACAGCAGCAGTCCTTCTTCGAACAAGGAGAGGAGTTCATGAAACCGATGGTACTGGCCGATATCGCCCAGGCCG
TCGAAATGCATGAATCCACTATTTCACGCGTGACCACCCAAAAATATCTGCACAGTCCGCGCGGTATTTTTGAGCTGAAA
TATTTCTTTTCCAGCCACGTCAATACAGAGGGCGGCGGCGAGGCATCGTCGACGGCCATTCGGGCGCTGGTGAAGAAGTT
AATCGCCGCGGAGAATCCCGCGAAGCCGTTGAGTGACAGTAAGCTGACCACCCTCCTGTCTGAACAGGGTATTATGGTGG
CACGTCGTACCGTCGCTAAGTACCGAGAGTCTTTATCCATTCCGCCATCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.257

100

0.48

Multiple sequence alignment