Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G3273_RS01360 Genome accession   NZ_CP048647
Coordinates   293955..295451 (+) Length   498 a.a.
NCBI ID   WP_001040640.1    Uniprot ID   -
Organism   Escherichia coli strain GW-AmxH19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 288955..300451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3273_RS01330 (G3273_01330) tusA 289202..289447 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  G3273_RS01335 (G3273_01335) zntA 289549..291747 (-) 2199 WP_001468058.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  G3273_RS01340 (G3273_01340) yhhN 291821..292447 (-) 627 WP_000964718.1 lysoplasmalogenase -
  G3273_RS01345 (G3273_01345) yhhM 292588..292947 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  G3273_RS01350 (G3273_01350) yhhL 292950..293219 (-) 270 WP_001295207.1 DUF1145 family protein -
  G3273_RS01355 (G3273_01355) rsmD 293209..293805 (-) 597 WP_000743189.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  G3273_RS01360 (G3273_01360) pilA 293955..295451 (+) 1497 WP_001040640.1 signal recognition particle-docking protein FtsY Machinery gene
  G3273_RS01365 (G3273_01365) ftsE 295454..296122 (+) 669 WP_042050926.1 cell division ATP-binding protein FtsE -
  G3273_RS01370 (G3273_01370) ftsX 296115..297173 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  G3273_RS01375 (G3273_01375) rpoH 297418..298272 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  G3273_RS01380 (G3273_01380) dapA 298376..299260 (-) 885 WP_000661262.1 4-hydroxy-tetrahydrodipicolinate synthase -
  G3273_RS01385 (G3273_01385) - 299260..300180 (-) 921 WP_001332164.1 phosphoglycerate dehydrogenase -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54600.34 Da        Isoelectric Point: 4.1714

>NTDB_id=370217 G3273_RS01360 WP_001040640.1 293955..295451(+) (pilA) [Escherichia coli strain GW-AmxH19]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLSEDVNAEAVSPEEWQAEAETVDIVEAAEEEAAKEEITDEEPEAQAL
AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=370217 G3273_RS01360 WP_001040640.1 293955..295451(+) (pilA) [Escherichia coli strain GW-AmxH19]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGATATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGATGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACCATCGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.571

77.309

0.375

Multiple sequence alignment