Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G3M73_RS17300 Genome accession   NZ_CP048605
Coordinates   3711693..3713126 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain PapRG-06-5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3706693..3718126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M73_RS17265 (G3M73_17265) elbB 3707384..3708037 (+) 654 WP_001302019.1 isoprenoid biosynthesis glyoxalase ElbB -
  G3M73_RS17270 (G3M73_17270) mtgA 3708034..3708762 (+) 729 WP_038339230.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  G3M73_RS17275 (G3M73_17275) yrbL 3708759..3709391 (-) 633 WP_053273571.1 PhoP regulatory network protein YrbL -
  G3M73_RS17280 (G3M73_17280) npr 3709605..3709877 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  G3M73_RS17285 (G3M73_17285) rapZ 3709874..3710728 (-) 855 WP_000243741.1 RNase adapter RapZ -
  G3M73_RS17290 (G3M73_17290) ptsN 3710774..3711265 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  G3M73_RS17295 (G3M73_17295) hpf 3711383..3711670 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  G3M73_RS17300 (G3M73_17300) rpoN/rpoN1 3711693..3713126 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  G3M73_RS17305 (G3M73_17305) lptB 3713174..3713899 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  G3M73_RS17310 (G3M73_17310) lptA 3713906..3714463 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  G3M73_RS17315 (G3M73_17315) lptC 3714432..3715007 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  G3M73_RS17320 (G3M73_17320) kdsC 3715004..3715570 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  G3M73_RS17325 (G3M73_17325) kdsD 3715591..3716577 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  G3M73_RS17330 (G3M73_17330) yrbG 3716591..3717568 (-) 978 WP_105517638.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=369978 G3M73_RS17300 WP_000809051.1 3711693..3713126(-) (rpoN/rpoN1) [Escherichia coli strain PapRG-06-5]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=369978 G3M73_RS17300 WP_000809051.1 3711693..3713126(-) (rpoN/rpoN1) [Escherichia coli strain PapRG-06-5]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTACCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTAACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAATAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAAGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465

Multiple sequence alignment