Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G3M73_RS16130 Genome accession   NZ_CP048605
Coordinates   3478471..3479967 (+) Length   498 a.a.
NCBI ID   WP_163425365.1    Uniprot ID   -
Organism   Escherichia coli strain PapRG-06-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3473471..3484967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M73_RS16100 (G3M73_16100) tusA 3473718..3473963 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  G3M73_RS16105 (G3M73_16105) zntA 3474065..3476263 (-) 2199 WP_028132010.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  G3M73_RS16110 (G3M73_16110) yhhN 3476337..3476963 (-) 627 WP_000964718.1 lysoplasmalogenase -
  G3M73_RS16115 (G3M73_16115) yhhM 3477104..3477463 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  G3M73_RS16120 (G3M73_16120) yhhL 3477466..3477735 (-) 270 WP_001295207.1 DUF1145 family protein -
  G3M73_RS16125 (G3M73_16125) rsmD 3477725..3478321 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  G3M73_RS16130 (G3M73_16130) pilA 3478471..3479967 (+) 1497 WP_163425365.1 signal recognition particle-docking protein FtsY Machinery gene
  G3M73_RS16135 (G3M73_16135) ftsE 3479970..3480638 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  G3M73_RS16140 (G3M73_16140) ftsX 3480631..3481689 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  G3M73_RS16145 (G3M73_16145) rpoH 3481934..3482788 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  G3M73_RS16150 (G3M73_16150) livJ 3483059..3484162 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  G3M73_RS16155 (G3M73_16155) ataR 3484312..3484578 (+) 267 WP_001277109.1 type II toxin-antitoxin system antitoxin AtaR -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54683.51 Da        Isoelectric Point: 4.1487

>NTDB_id=369972 G3M73_RS16130 WP_163425365.1 3478471..3479967(+) (pilA) [Escherichia coli strain PapRG-06-5]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAAEVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=369972 G3M73_RS16130 WP_163425365.1 3478471..3479967(+) (pilA) [Escherichia coli strain PapRG-06-5]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGAAGTTGTGGAAGTCACTGAACAGGTAGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAGCGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCAGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCAACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTTTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGC
CGTTTGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCAGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAGCGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment