Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   D073_RS17625 Genome accession   NZ_CP041145
Coordinates   3551377..3552522 (+) Length   381 a.a.
NCBI ID   WP_020957973.1    Uniprot ID   -
Organism   Bacillus velezensis strain At1     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3546377..3557522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D073_RS17600 (D073_3338) - 3547274..3547702 (-) 429 WP_007407634.1 DUF1934 domain-containing protein -
  D073_RS17605 (D073_3339) - 3547856..3548368 (+) 513 Protein_3383 bacteriocin biosynthesis protein AlbG -
  D073_RS17610 (D073_3340) - 3548375..3549751 (-) 1377 WP_020957971.1 YncE family protein -
  D073_RS17615 (D073_3341) - 3549791..3551146 (-) 1356 WP_020957972.1 YncE family protein -
  D073_RS17625 (D073_3342) rapF 3551377..3552522 (+) 1146 WP_020957973.1 tetratricopeptide repeat protein Regulator
  D073_RS17630 phrF 3552506..3552625 (+) 120 WP_041482476.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  D073_RS17635 (D073_3343) - 3552823..3553026 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  D073_RS17640 (D073_3344) - 3553040..3553243 (+) 204 WP_007407641.1 hypothetical protein -
  D073_RS17645 (D073_3345) - 3553278..3553463 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  D073_RS17650 - 3553649..3553930 (+) 282 WP_007407643.1 hypothetical protein -
  D073_RS17655 (D073_3346) - 3553974..3554486 (+) 513 WP_039253458.1 stage II sporulation protein M -
  D073_RS17665 - 3555077..3555295 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  D073_RS17670 (D073_3348) speB 3555463..3556335 (-) 873 WP_020957975.1 agmatinase -
  D073_RS17675 (D073_3349) speE 3556394..3557224 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45587.69 Da        Isoelectric Point: 4.5769

>NTDB_id=369856 D073_RS17625 WP_020957973.1 3551377..3552522(+) (rapF) [Bacillus velezensis strain At1]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIAEYEFLESLYVGEPDEEAI
MECFDFLRDKMMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=369856 D073_RS17625 WP_020957973.1 3551377..3552522(+) (rapF) [Bacillus velezensis strain At1]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCATAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCAGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCACAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATCGCCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAAATGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

56.579

99.738

0.564

Multiple sequence alignment