Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G3M71_RS01230 Genome accession   NZ_CP048603
Coordinates   273313..274809 (+) Length   498 a.a.
NCBI ID   WP_001040638.1    Uniprot ID   -
Organism   Escherichia coli strain ChlosBP-23-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 268313..279809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M71_RS01200 (G3M71_01200) tusA 268393..268638 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  G3M71_RS01205 (G3M71_01205) zntA 268907..271105 (-) 2199 WP_163388356.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  G3M71_RS01210 (G3M71_01210) yhhN 271179..271805 (-) 627 WP_000964718.1 lysoplasmalogenase -
  G3M71_RS01215 (G3M71_01215) yhhM 271946..272305 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  G3M71_RS01220 (G3M71_01220) yhhL 272308..272577 (-) 270 WP_001295207.1 DUF1145 family protein -
  G3M71_RS01225 (G3M71_01225) rsmD 272567..273163 (-) 597 WP_000743189.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  G3M71_RS01230 (G3M71_01230) pilA 273313..274809 (+) 1497 WP_001040638.1 signal recognition particle-docking protein FtsY Machinery gene
  G3M71_RS01235 (G3M71_01235) ftsE 274812..275480 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  G3M71_RS01240 (G3M71_01240) ftsX 275473..276531 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  G3M71_RS01245 (G3M71_01245) rpoH 276776..277630 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  G3M71_RS01250 (G3M71_01250) livJ 277901..279004 (+) 1104 WP_001562228.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  G3M71_RS01255 (G3M71_01255) panM 279192..279575 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54610.38 Da        Isoelectric Point: 4.1714

>NTDB_id=369761 G3M71_RS01230 WP_001040638.1 273313..274809(+) (pilA) [Escherichia coli strain ChlosBP-23-1]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKDEITDEEPEAQAL
AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=369761 G3M71_RS01230 WP_001040638.1 273313..274809(+) (pilA) [Escherichia coli strain ChlosBP-23-1]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGACGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.705

77.51

0.378

Multiple sequence alignment