Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   D069_RS18320 Genome accession   NZ_CP041143
Coordinates   3640856..3642001 (+) Length   381 a.a.
NCBI ID   WP_007407638.1    Uniprot ID   -
Organism   Bacillus velezensis strain UCMB5007     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3635856..3647001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D069_RS18295 (D069_3424) - 3636757..3637185 (-) 429 WP_032869448.1 DUF1934 domain-containing protein -
  D069_RS18300 - 3637339..3637847 (+) 509 Protein_3527 bacteriocin biosynthesis protein AlbG -
  D069_RS18305 (D069_3425) - 3637854..3639230 (-) 1377 WP_105937194.1 YncE family protein -
  D069_RS18310 (D069_3426) - 3639270..3640625 (-) 1356 WP_105937193.1 YncE family protein -
  D069_RS18320 (D069_3427) rapF 3640856..3642001 (+) 1146 WP_007407638.1 tetratricopeptide repeat protein Regulator
  D069_RS18325 - 3641985..3642104 (+) 120 WP_021494790.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  D069_RS18330 (D069_3428) - 3642302..3642505 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  D069_RS18335 (D069_3429) - 3642519..3642722 (+) 204 WP_007407641.1 hypothetical protein -
  D069_RS18340 (D069_3430) - 3642757..3642942 (+) 186 WP_140957686.1 DUF4177 domain-containing protein -
  D069_RS18345 - 3643128..3643409 (+) 282 WP_007407643.1 hypothetical protein -
  D069_RS18350 (D069_3431) - 3643453..3643965 (+) 513 WP_140957688.1 stage II sporulation protein M -
  D069_RS18360 - 3644556..3644774 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  D069_RS18365 (D069_3433) speB 3644942..3645814 (-) 873 WP_007614448.1 agmatinase -
  D069_RS18370 (D069_3434) speE 3645873..3646703 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45543.57 Da        Isoelectric Point: 4.5769

>NTDB_id=369706 D069_RS18320 WP_007407638.1 3640856..3642001(+) (rapF) [Bacillus velezensis strain UCMB5007]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIAEYEFLESLYVGEPDEEAI
MECFDFLRDKSMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=369706 D069_RS18320 WP_007407638.1 3640856..3642001(+) (rapF) [Bacillus velezensis strain UCMB5007]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGCATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATCGCCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATCGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.478

100

0.785
  rapC Bacillus subtilis subsp. subtilis str. 168

56.579

99.738

0.564

Multiple sequence alignment