Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   FJM50_RS23315 Genome accession   NZ_CP041066
Coordinates   4273749..4274123 (+) Length   124 a.a.
NCBI ID   WP_223269005.1    Uniprot ID   -
Organism   Priestia megaterium strain KNU-01     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4268749..4279123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJM50_RS23295 - 4269239..4269910 (-) 672 WP_013058558.1 response regulator transcription factor -
  FJM50_RS23300 - 4270236..4271054 (+) 819 WP_013058559.1 squalene/phytoene synthase family protein -
  FJM50_RS23305 - 4271091..4273040 (-) 1950 WP_064472035.1 FUSC family protein -
  FJM50_RS23310 - 4273196..4273456 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  FJM50_RS23315 nucA/comI 4273749..4274123 (+) 375 WP_223269005.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  FJM50_RS23320 comJ 4274159..4274566 (+) 408 WP_064472036.1 competence protein ComJ -
  FJM50_RS23325 helD 4274595..4276913 (-) 2319 WP_064472037.1 RNA polymerase recycling motor HelD -
  FJM50_RS30430 - 4277158..4277283 (-) 126 WP_016765178.1 hypothetical protein -
  FJM50_RS23330 - 4277423..4277941 (-) 519 WP_223268980.1 enoyl-CoA hydratase/isomerase family protein -
  FJM50_RS23335 - 4278181..4278549 (+) 369 WP_029323759.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13661.90 Da        Isoelectric Point: 4.2601

>NTDB_id=369316 FJM50_RS23315 WP_223269005.1 4273749..4274123(+) (nucA/comI) [Priestia megaterium strain KNU-01]
MGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=369316 FJM50_RS23315 WP_223269005.1 4273749..4274123(+) (nucA/comI) [Priestia megaterium strain KNU-01]
ATGGGACATGCGGCGTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAACTACGATGCGACGCTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGGGAAGAATCGCTGAAAGGATATCCGACAAAAACAGGCTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGCGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.227

95.968

0.645

Multiple sequence alignment