Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   FJM50_RS20430 Genome accession   NZ_CP041066
Coordinates   3760389..3760811 (+) Length   140 a.a.
NCBI ID   WP_140456946.1    Uniprot ID   -
Organism   Priestia megaterium strain KNU-01     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3755389..3765811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJM50_RS20405 - 3755720..3756553 (-) 834 WP_098288094.1 AraC family transcriptional regulator -
  FJM50_RS20410 - 3756777..3757598 (+) 822 WP_053487481.1 sensor histidine kinase -
  FJM50_RS20415 - 3757599..3758240 (+) 642 WP_057273553.1 response regulator transcription factor -
  FJM50_RS20420 - 3758372..3759754 (+) 1383 WP_082611746.1 PepSY domain-containing protein -
  FJM50_RS20425 - 3759784..3760248 (+) 465 WP_140456945.1 FixH family protein -
  FJM50_RS20430 nucA/comI 3760389..3760811 (+) 423 WP_140456946.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  FJM50_RS20435 - 3760965..3761318 (+) 354 WP_223272376.1 DUF1516 family protein -
  FJM50_RS20440 - 3761359..3761952 (-) 594 WP_013057951.1 hypothetical protein -
  FJM50_RS20445 - 3762108..3762764 (-) 657 WP_029322917.1 TetR/AcrR family transcriptional regulator -
  FJM50_RS20450 - 3762788..3764068 (-) 1281 WP_228540830.1 glycosyltransferase -
  FJM50_RS20455 - 3764366..3764911 (+) 546 WP_064471863.1 GNAT family N-acetyltransferase -
  FJM50_RS20460 - 3765211..3765696 (-) 486 WP_029322922.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15310.13 Da        Isoelectric Point: 4.4982

>NTDB_id=369311 FJM50_RS20430 WP_140456946.1 3760389..3760811(+) (nucA/comI) [Priestia megaterium strain KNU-01]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADHYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=369311 FJM50_RS20430 WP_140456946.1 3760389..3760811(+) (nucA/comI) [Priestia megaterium strain KNU-01]
TTGAAAAAAATTGTTTCTGGAATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCATTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGGGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCCGATAT
TGAATATGTAAAGCCTTCAGACAATCGAGGCGCCGGGTCTTGGATCTCACATCAAGTAGACGAGTTTCCTGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

60.345

82.857

0.5

Multiple sequence alignment