Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FJM50_RS04480 Genome accession   NZ_CP041066
Coordinates   597222..598598 (+) Length   458 a.a.
NCBI ID   WP_013054899.1    Uniprot ID   A0A8D4J4E8
Organism   Priestia megaterium strain KNU-01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 592222..603598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJM50_RS04460 - 592514..592975 (+) 462 WP_013054895.1 CtsR family transcriptional regulator -
  FJM50_RS04465 - 593017..593559 (+) 543 WP_013054896.1 UvrB/UvrC motif-containing protein -
  FJM50_RS04470 - 593563..594636 (+) 1074 WP_013054897.1 protein arginine kinase -
  FJM50_RS04475 clpC 594657..597098 (+) 2442 WP_014462041.1 ATP-dependent protease ATP-binding subunit ClpC -
  FJM50_RS04480 radA 597222..598598 (+) 1377 WP_013054899.1 DNA repair protein RadA Machinery gene
  FJM50_RS04485 disA 598602..599681 (+) 1080 WP_013054900.1 DNA integrity scanning diadenylate cyclase DisA -
  FJM50_RS04490 - 599829..600926 (+) 1098 WP_013054901.1 PIN/TRAM domain-containing protein -
  FJM50_RS04495 ispD 600952..601653 (+) 702 WP_029325232.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  FJM50_RS04500 ispF 601650..602129 (+) 480 WP_013054903.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49474.82 Da        Isoelectric Point: 6.9049

>NTDB_id=369285 FJM50_RS04480 WP_013054899.1 597222..598598(+) (radA) [Priestia megaterium strain KNU-01]
MAKRKTKFACQHCGYESAKWMGKCPGCGSWNSMVEEMEETKSSRRGAFSGATASKVQKPQSITAIESTTEPRIFTPSAEL
NRVLGGGIVRGSLVLIGGDPGIGKSTLLLQTSAQLAMKQNKVLYISGEESTKQTKLRADRLGVKAEELYVHAETNLELIL
EAISSMQPDFVVIDSIQTIYHADVTSAPGSVSQVRECTAELMRVAKTNGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPTSF
GNPRRMATGVDHNRVSLIMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDAASSATDVIVGEVGLT
GEVRRVSRIEQRVQEAVKLGFQRIIIPEKNLGGWKVPDGIDVIGVSTVAEALQYTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=369285 FJM50_RS04480 WP_013054899.1 597222..598598(+) (radA) [Priestia megaterium strain KNU-01]
ATGGCGAAACGAAAAACAAAATTTGCATGTCAGCATTGCGGATATGAATCAGCAAAATGGATGGGGAAATGTCCCGGATG
CGGAAGCTGGAATTCTATGGTAGAAGAAATGGAAGAAACGAAATCATCACGCCGAGGAGCTTTTTCCGGAGCTACTGCTT
CTAAAGTTCAAAAACCTCAGTCAATTACAGCGATTGAATCTACAACAGAACCACGAATTTTTACACCTTCTGCAGAGTTA
AATCGTGTATTAGGAGGAGGCATTGTGAGAGGGTCACTCGTTTTAATAGGGGGAGATCCGGGTATCGGAAAGTCTACTTT
ACTTTTGCAAACCTCTGCGCAGTTAGCAATGAAACAAAATAAAGTACTATATATTTCGGGAGAGGAATCAACCAAACAAA
CGAAGTTAAGAGCGGACAGGCTAGGTGTAAAAGCTGAAGAGCTTTATGTTCACGCAGAAACAAACTTAGAATTAATACTA
GAGGCCATCTCAAGCATGCAGCCTGATTTTGTGGTGATTGATTCAATTCAAACCATTTACCATGCTGATGTGACATCAGC
GCCAGGAAGCGTCTCACAGGTGCGAGAGTGTACGGCTGAACTAATGCGTGTTGCTAAAACGAATGGAATTGCCATCTTTA
TTGTGGGTCATGTAACGAAAGAAGGGGCCATTGCAGGTCCAAGACTTCTTGAACATATGGTGGATACGGTTCTTTATTTT
GAAGGAGAGCGCCATCATACGTATCGTATATTAAGAGCGGTTAAGAACCGGTTTGGATCTACAAATGAAATGGGCATTTT
TGAAATGAAAGAAAGTGGGCTAGAAGAAGTATTGAATCCATCTGAAATCTTTCTAGAAGAACGATCTCAGGGAGCAGCAG
GATCGGTTGTTGTCGCTTCAATGGAAGGGACAAGACCCGTATTAGTAGAGCTGCAGGCGCTAATCAGTCCAACGAGCTTT
GGAAACCCTAGAAGAATGGCGACGGGAGTAGATCATAATCGCGTTTCGCTTATCATGGCTGTCTTAGAAAAGCGTGTAGG
AATGCTGCTGCAGAATCAAGATGCTTATTTAAAAGTAGCAGGTGGAGTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TAGCCGTTAGCATTGCTTCAAGCTTCAGAGATGCTGCTTCTAGCGCTACAGATGTTATTGTAGGAGAGGTAGGATTAACA
GGTGAAGTACGCCGAGTGTCTAGAATTGAACAGCGCGTTCAAGAAGCGGTAAAATTAGGGTTCCAACGAATTATTATCCC
GGAGAAAAATTTAGGAGGATGGAAAGTCCCAGATGGTATTGATGTGATTGGGGTATCAACAGTAGCGGAGGCTCTGCAAT
ATACATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.817

100

0.76
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

63.135

98.908

0.624

Multiple sequence alignment