Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   FJM75_RS21630 Genome accession   NZ_CP041063
Coordinates   4216474..4219965 (+) Length   1163 a.a.
NCBI ID   WP_166001368.1    Uniprot ID   -
Organism   Bacillus sp. Cs-700     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4211474..4224965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJM75_RS21595 - 4211894..4213144 (+) 1251 WP_166001364.1 hypothetical protein -
  FJM75_RS21600 - 4213375..4213830 (+) 456 WP_242688510.1 pyridoxamine 5'-phosphate oxidase family protein -
  FJM75_RS21605 - 4213933..4214559 (+) 627 WP_098443058.1 TVP38/TMEM64 family protein -
  FJM75_RS21610 cotJC 4214910..4215479 (-) 570 WP_098443059.1 spore coat protein CotJC -
  FJM75_RS21615 - 4215501..4215767 (-) 267 WP_098443060.1 spore coat protein CotJB -
  FJM75_RS21620 - 4215771..4215986 (-) 216 WP_098443061.1 spore coat associated protein CotJA -
  FJM75_RS21625 - 4216098..4216292 (-) 195 WP_098443062.1 hypothetical protein -
  FJM75_RS21630 addB 4216474..4219965 (+) 3492 WP_166001368.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  FJM75_RS21635 addA 4219955..4223668 (+) 3714 WP_166001370.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  FJM75_RS21640 - 4223731..4224891 (+) 1161 WP_166001372.1 exonuclease SbcCD subunit D -

Sequence

Protein


Download         Length: 1163 a.a.        Molecular weight: 134194.93 Da        Isoelectric Point: 5.2421

>NTDB_id=369263 FJM75_RS21630 WP_166001368.1 4216474..4219965(+) (addB) [Bacillus sp. Cs-700]
MTVKFVIGRSGSGKSNHCLTEMQNELTDRPIGQDMIYLVPEQMTFQSEYELVHNRGLKGMIRAQVFSFTRLAWKVLQETG
GMARPHLSSVGTSMMLRQIIEENKQQLRIYQRSSEKTGYVEQLNDMVTEFKRYCLEPDDIKSFAEDFYVEDERNLLRDKL
YDLHIVYEAFQKKMIGHYLDSEDYLALLAEKIEESDYLANATIWIDGFHSFTPQELLVIQSLMKKGTTLTFTITMDSEMI
PSSIHELDLFHEPGKTYQILQGLANEEGVEIEEPLILRTQKRYQNSEISHLEANYGERPPVVYRGELQHIQVHHAVNIRS
EVEAVAREALRLVRDEGYRYRDISIMVRNMSTYYELVETIFEDHGIPIFSDQKRTMLHHPIIELIRSSLDIIQYNWRYES
VFRCVKTELLFPDDTGESIETIRERMDELENYVIAKGIKGYRWKTGERFKANIYRQLEDKEVITSDELEKMEDRLNETKD
WIATPLEKFERHMTKAKTVEEMCEILYYLLENCHAAEKIDRLKEQAEKAGHLAKAREHDQVWDAVISLLDETVELIGDQK
LSVEQFTKLLEAGMENMKFALVPASLDQVVIGSIDRTRFTDVKCGFLLGVNEGVLPARIQEDGLLSEQDREKLEEQGIQL
APGLKRRLLDEEFLIYHSLATPSDSLWVSCPLADEEGKGLQTSIVMNRLKEMFPDLKMGLKFAEPGEEATEELSFITNPS
KTIGRLTGQLRQWHRGYPIDPIWWDAYNWFISAPTEKDRVRLLDSLFYRNKAERLTESTSRKLYGHQIQTSVSRMERYKS
CAFSQFASHGLRLKERATFKLEAPDIGQLFHAALNMMAETIKQRHWDWAKLSSNQYYELSGQIVEQLAPKLQNEILLSSN
RYHYIMRKLTQVVGRASSVLGQQAKKSGFQPAGLELGFGPNQELPPIEFTLKNGVKIQLIGRVDRVDQALSGEELLLRII
DYKSSGTSLDLSEVYFGLALQMLTYLDVVISNANVWLGREATAAGMLYFHVHNPMLQTSAPGQAAIQQELFRKFKMKGLL
LADKDVVSLMDNDIETKKSDIIPAALKKDGSFYKNSSVLSSDDFHALRSYTRKTIQSIGTEISDGRIEIDPYKMKDRIPC
TFCSYRSFCQFDQAMEDNAYRPITAQDDETILKRMREEADDDN

Nucleotide


Download         Length: 3492 bp        

>NTDB_id=369263 FJM75_RS21630 WP_166001368.1 4216474..4219965(+) (addB) [Bacillus sp. Cs-700]
ATGACAGTGAAATTTGTCATTGGTCGTTCAGGTAGTGGGAAATCGAATCATTGTTTAACTGAAATGCAAAATGAACTAAC
CGATCGGCCAATCGGACAGGATATGATTTACCTCGTTCCTGAGCAAATGACGTTTCAATCAGAGTATGAATTAGTACATA
ATAGAGGTTTAAAAGGAATGATTCGAGCGCAGGTTTTTAGTTTTACAAGACTTGCCTGGAAAGTGCTTCAAGAAACTGGA
GGGATGGCTCGTCCCCATTTAAGTAGTGTTGGTACGAGTATGATGCTTCGACAGATCATTGAGGAAAATAAGCAGCAGCT
ACGAATTTATCAGCGGTCTTCTGAGAAAACAGGATACGTGGAACAGCTAAATGATATGGTAACGGAATTTAAGCGGTATT
GTCTCGAGCCAGATGATATCAAGAGCTTTGCTGAAGATTTTTATGTAGAAGATGAGCGGAACTTACTTCGAGATAAGCTC
TATGACTTGCACATTGTTTACGAAGCTTTTCAAAAGAAGATGATAGGTCACTACCTCGATTCAGAAGACTATTTAGCGCT
GCTGGCAGAGAAAATTGAGGAATCAGACTATCTAGCGAACGCTACAATTTGGATAGACGGCTTTCATAGTTTTACTCCTC
AGGAACTACTGGTCATTCAATCTTTAATGAAAAAAGGAACAACCCTTACGTTTACGATTACGATGGATTCTGAAATGATC
CCTTCATCTATTCATGAACTCGACCTGTTTCATGAACCAGGTAAAACCTATCAAATCCTTCAGGGACTGGCGAATGAAGA
AGGGGTAGAAATCGAGGAACCTCTCATATTAAGAACGCAGAAGAGGTATCAAAACAGTGAGATCTCTCATTTAGAAGCCA
ATTATGGTGAACGGCCGCCGGTTGTTTATCGTGGGGAGTTGCAGCACATACAAGTTCATCATGCGGTGAATATACGTTCT
GAAGTTGAGGCAGTAGCACGAGAAGCGCTAAGGCTTGTGCGAGATGAAGGTTATCGTTACAGAGATATTTCAATCATGGT
ACGCAATATGTCAACTTATTATGAACTTGTGGAAACGATATTCGAGGATCACGGGATTCCCATTTTCTCAGATCAAAAGC
GGACAATGCTTCACCACCCGATAATTGAGTTAATTCGCTCAAGTTTGGACATTATCCAATATAACTGGCGCTATGAATCA
GTTTTTCGGTGTGTCAAAACGGAACTATTGTTTCCAGACGATACGGGAGAAAGTATTGAAACCATTCGTGAACGGATGGA
TGAACTTGAGAATTACGTTATAGCAAAAGGCATCAAAGGTTATCGTTGGAAAACTGGTGAAAGATTTAAAGCAAATATTT
ATCGTCAGCTAGAAGATAAAGAAGTTATCACTTCCGATGAACTCGAAAAAATGGAAGATCGCCTGAATGAAACGAAAGAC
TGGATTGCGACGCCGCTTGAGAAATTTGAGCGTCACATGACCAAAGCAAAAACCGTTGAAGAAATGTGCGAAATTCTTTA
TTATCTTCTTGAAAATTGCCACGCAGCGGAAAAAATCGATCGTTTAAAAGAGCAGGCAGAAAAGGCCGGCCACCTTGCCA
AAGCACGTGAACATGATCAGGTTTGGGACGCGGTGATTTCGCTACTTGATGAAACAGTGGAATTAATCGGTGATCAGAAA
CTCTCCGTGGAGCAATTTACAAAGTTGCTTGAAGCCGGAATGGAGAATATGAAATTTGCTCTCGTACCTGCCTCACTTGA
CCAGGTAGTGATTGGAAGCATTGATCGTACAAGATTTACTGATGTAAAATGTGGCTTTCTTCTAGGTGTAAATGAAGGTG
TCTTACCGGCTCGCATTCAGGAAGATGGACTGTTAAGTGAGCAGGATAGAGAAAAGTTAGAAGAACAGGGAATTCAGTTA
GCACCTGGGTTGAAGCGGCGTTTGCTTGATGAAGAATTTTTGATTTACCATTCGCTAGCTACACCTTCGGATTCTTTATG
GGTTAGCTGTCCACTTGCTGATGAAGAAGGAAAGGGTCTGCAAACTTCTATCGTCATGAATCGATTGAAGGAGATGTTCC
CGGATCTTAAGATGGGACTGAAATTTGCAGAGCCTGGTGAAGAAGCAACGGAAGAACTTTCTTTCATAACAAACCCATCC
AAGACAATAGGGCGACTCACGGGACAGCTTCGACAATGGCACCGAGGGTATCCGATTGATCCTATTTGGTGGGATGCATA
CAATTGGTTTATCTCGGCTCCAACTGAGAAAGATCGCGTACGCTTACTTGATAGTCTTTTTTATCGAAATAAAGCCGAAC
GTCTAACTGAATCAACGAGTAGAAAACTTTATGGTCACCAAATTCAGACGAGTGTTTCAAGAATGGAAAGATATAAATCT
TGTGCTTTCTCTCAGTTTGCTTCTCATGGTTTACGCTTAAAAGAACGAGCGACATTCAAGCTTGAAGCGCCTGATATCGG
ACAGCTGTTTCATGCGGCGCTCAATATGATGGCCGAAACGATTAAGCAGCGTCACTGGGATTGGGCTAAACTCTCATCTA
ATCAATATTATGAACTTTCAGGACAAATCGTTGAACAGCTCGCACCAAAGTTACAGAATGAAATTCTATTAAGTTCAAAT
CGCTATCATTATATTATGAGAAAGCTAACGCAAGTTGTTGGCCGCGCCTCGTCCGTTCTTGGGCAACAGGCAAAGAAAAG
CGGGTTTCAACCAGCAGGACTTGAATTAGGATTTGGACCCAATCAAGAGCTCCCTCCAATCGAGTTCACGCTTAAAAATG
GAGTTAAAATCCAGCTCATCGGACGTGTCGATCGTGTTGATCAGGCACTGAGCGGTGAAGAGCTTCTCCTTCGTATTATC
GACTATAAGTCTAGCGGTACTTCTCTTGACCTATCGGAAGTGTATTTTGGACTCGCTCTACAAATGCTGACGTATCTCGA
TGTTGTTATATCCAATGCGAACGTCTGGCTTGGAAGAGAGGCAACTGCTGCAGGTATGCTTTATTTTCATGTCCATAACC
CTATGCTCCAGACGTCAGCGCCTGGACAAGCGGCAATTCAACAGGAGCTTTTCAGGAAATTTAAGATGAAGGGACTTCTC
CTTGCGGATAAAGACGTTGTGAGCTTAATGGATAACGACATTGAAACGAAGAAATCGGATATTATCCCTGCCGCACTGAA
AAAGGATGGTAGCTTTTACAAAAATTCTTCCGTCTTATCTTCTGATGATTTCCATGCGCTTCGTTCTTATACAAGAAAAA
CGATTCAATCTATTGGAACAGAAATTTCAGATGGCCGTATTGAGATAGATCCTTACAAAATGAAAGATCGAATTCCATGT
ACGTTCTGTTCATACCGTTCATTTTGCCAGTTTGATCAGGCGATGGAAGATAATGCTTATCGTCCTATTACTGCTCAGGA
TGACGAAACGATTTTAAAACGCATGAGAGAGGAGGCAGACGATGATAACTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.102

100

0.494

Multiple sequence alignment