Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GZH78_RS28135 Genome accession   NZ_CP048408
Coordinates   6239147..6240661 (-) Length   504 a.a.
NCBI ID   WP_163976721.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain DR397     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6234147..6245661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH78_RS28105 (GZH78_28115) thiS 6234965..6235165 (-) 201 WP_007953386.1 sulfur carrier protein ThiS -
  GZH78_RS28110 (GZH78_28120) - 6235238..6235618 (-) 381 WP_163976719.1 DUF423 domain-containing protein -
  GZH78_RS28115 (GZH78_28125) mtgA 6235693..6236415 (+) 723 WP_103385340.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  GZH78_RS28120 (GZH78_28130) rpoH 6236487..6237341 (-) 855 WP_007953393.1 RNA polymerase sigma factor RpoH -
  GZH78_RS28125 (GZH78_28135) ftsX 6237457..6238482 (-) 1026 WP_007953395.1 permease-like cell division protein FtsX -
  GZH78_RS28130 (GZH78_28140) ftsE 6238479..6239150 (-) 672 WP_007953396.1 cell division ATP-binding protein FtsE -
  GZH78_RS28135 (GZH78_28145) pilA 6239147..6240661 (-) 1515 WP_163976721.1 signal recognition particle-docking protein FtsY Machinery gene
  GZH78_RS28140 (GZH78_28150) - 6240660..6240854 (+) 195 WP_039767922.1 hypothetical protein -
  GZH78_RS28145 (GZH78_28155) - 6241006..6242361 (+) 1356 WP_085605895.1 pitrilysin family protein -
  GZH78_RS28150 (GZH78_28160) - 6242354..6243841 (+) 1488 WP_039767920.1 pitrilysin family protein -
  GZH78_RS28155 (GZH78_28165) rsmD 6243841..6244446 (+) 606 WP_085611985.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -

Sequence

Protein


Download         Length: 504 a.a.        Molecular weight: 52895.13 Da        Isoelectric Point: 4.6508

>NTDB_id=368643 GZH78_RS28135 WP_163976721.1 6239147..6240661(-) (pilA) [Pseudomonas fluorescens strain DR397]
MFGSNDDKKTPAAAGEKKSLFGWLRKKPQEPVVEQPTAIPEPAPAPAPVIEEAPAPVVLPIAEPVLQPVVEAEPEAPAAA
ELPLSPASEPWLTLPVAEEPVALVEEAAPHVTPPIPAPVAFAPEPVQAPVVEAAPVIPEPVVAAPVAPIVEAPEPAPAPA
PAPAPAPAPVAVAPVAPVEIQVETPLEAPRTEETKAGFFARLKQGLSKTSASIGEGMASLFLGKKAIDDDLLDDLETRLL
TADVGVEATSVIIQRLTQKVARKELADSDALYKSLQAELAAMLKPVEQPLKIASQNKPFVILVVGVNGAGKTTTIGKLAK
KLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNKIPVIAQHTGADSASVIFDAVQAAKARGIDVLIADTAGRLHTKDNLME
ELKKVRRVIGKLDADAPHEVLLVLDAGTGQNAINQAKQFNQTVELTGLALTKLDGTAKGGVIFALAKQFGLPIRYIGVGE
GIDDLRTFEAEPFVQALFAERERS

Nucleotide


Download         Length: 1515 bp        

>NTDB_id=368643 GZH78_RS28135 WP_163976721.1 6239147..6240661(-) (pilA) [Pseudomonas fluorescens strain DR397]
ATGTTTGGTTCCAACGACGACAAGAAGACCCCAGCTGCGGCTGGCGAGAAGAAAAGCCTGTTCGGATGGCTGCGCAAGAA
ACCGCAGGAACCCGTCGTCGAACAGCCGACTGCCATTCCTGAGCCAGCCCCTGCGCCCGCTCCCGTAATAGAAGAAGCGC
CGGCGCCGGTTGTGCTGCCGATCGCCGAGCCGGTGCTGCAACCGGTGGTCGAGGCCGAGCCTGAAGCGCCGGCTGCCGCC
GAATTGCCGCTGTCCCCGGCCAGCGAGCCGTGGCTGACCTTGCCGGTGGCGGAAGAGCCGGTGGCGCTGGTTGAAGAGGC
TGCGCCGCACGTTACACCGCCGATTCCTGCACCGGTCGCGTTCGCCCCCGAACCTGTTCAGGCGCCGGTGGTGGAAGCTG
CTCCGGTCATTCCAGAACCTGTGGTTGCTGCCCCGGTTGCTCCGATTGTTGAAGCGCCCGAGCCTGCACCTGCACCTGCA
CCTGCACCTGCACCTGCACCTGCACCTGTCGCCGTAGCGCCAGTCGCTCCTGTGGAAATCCAGGTTGAAACCCCGTTAGA
GGCCCCGCGCACCGAAGAAACCAAGGCCGGCTTCTTCGCCCGTCTCAAGCAGGGCCTGTCCAAGACCAGTGCCAGCATCG
GCGAGGGCATGGCCAGCCTGTTCCTCGGCAAGAAAGCCATCGATGACGATCTGCTCGACGATCTCGAAACCCGCCTGCTG
ACGGCCGACGTCGGCGTCGAGGCGACTTCGGTGATCATCCAGCGCCTGACCCAGAAGGTCGCCCGCAAGGAGCTGGCCGA
TTCCGACGCCCTGTACAAATCCCTGCAGGCCGAGTTGGCCGCAATGCTCAAACCGGTCGAGCAGCCGCTGAAGATCGCTT
CCCAGAACAAGCCATTCGTGATTCTGGTGGTTGGCGTCAACGGCGCCGGCAAGACCACCACCATCGGCAAACTGGCGAAG
AAACTGCAACTGGAAGGCAAGAAAGTCATGCTCGCTGCCGGTGACACCTTCCGTGCCGCGGCTGTCGAGCAGTTGCAGGT
CTGGGGCGAGCGCAACAAGATTCCGGTGATCGCCCAGCACACGGGCGCGGACTCGGCTTCGGTGATCTTCGACGCCGTGC
AGGCCGCCAAGGCCCGTGGCATTGATGTGCTGATCGCCGACACCGCCGGTCGTCTGCACACCAAAGACAACCTGATGGAA
GAACTGAAAAAGGTTCGCCGGGTGATCGGCAAGCTCGACGCCGACGCGCCGCACGAAGTGCTGCTGGTGCTCGACGCCGG
CACCGGCCAGAACGCCATCAACCAGGCCAAGCAATTCAACCAGACCGTCGAACTGACCGGCCTGGCGCTGACCAAGCTCG
ACGGCACCGCCAAGGGCGGGGTGATTTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGCGTCGGTGAA
GGCATCGACGATCTGCGTACCTTTGAAGCCGAACCCTTTGTCCAGGCACTGTTTGCCGAGCGGGAGCGTTCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(214-279)

SRP54-type protein, GTPase domain (SRP54)

(298-498)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.371

79.167

0.383

Multiple sequence alignment