Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GZH78_RS17295 Genome accession   NZ_CP048408
Coordinates   3908816..3910222 (-) Length   468 a.a.
NCBI ID   WP_163975527.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain DR397     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3903816..3915222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH78_RS17285 (GZH78_17290) - 3906147..3907256 (-) 1110 WP_163975525.1 substrate-binding domain-containing protein -
  GZH78_RS17290 (GZH78_17295) gspF 3907605..3908816 (-) 1212 WP_085654186.1 type II secretion system inner membrane protein GspF -
  GZH78_RS17295 (GZH78_17300) pilB 3908816..3910222 (-) 1407 WP_163975527.1 type II secretion system ATPase GspE Machinery gene
  GZH78_RS17300 (GZH78_17305) gspD 3910219..3912570 (-) 2352 WP_163975529.1 type II secretion system secretin GspD -
  GZH78_RS17305 (GZH78_17310) gspM 3912567..3913112 (-) 546 WP_163975531.1 type II secretion system protein GspM -
  GZH78_RS17310 (GZH78_17315) gspL 3913105..3914250 (-) 1146 WP_163975533.1 type II secretion system protein GspL -
  GZH78_RS17315 (GZH78_17320) gspK 3914247..3915200 (-) 954 WP_163975535.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 468 a.a.        Molecular weight: 50894.36 Da        Isoelectric Point: 6.0980

>NTDB_id=368595 GZH78_RS17295 WP_163975527.1 3908816..3910222(-) (pilB) [Pseudomonas fluorescens strain DR397]
MSALPYAWAKAQRILLRDGVLTVCPSTPGWSINEVRRQFGAARLERVRDDELDGLLATAYADTGSAAAVVGAAENEVDLD
RLMQDMPEITDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGTLRDVVSPRKALHGALVSRIKIM
AQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLHLETLGMDAQVLAKLDHLIRQPHGIVLV
TGPTGSGKTTSLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNAKIDMTFALALRAILRQDPDIIMIGEIRDLETAQ
IAVQASLTGHLVLATLHTNDAVSAVNRLIDMGVEPFLLASSMLGVLAQRLVRRLCNQCKQEDPATPGTWRPVGCAACNHT
GYSGRTGIHELFCIDDDIRTLIHQGAGEQALRAAASKAGMFSLREDGERWIRSGATAPEEILRVTRDA

Nucleotide


Download         Length: 1407 bp        

>NTDB_id=368595 GZH78_RS17295 WP_163975527.1 3908816..3910222(-) (pilB) [Pseudomonas fluorescens strain DR397]
ATGAGCGCATTGCCTTACGCCTGGGCCAAGGCGCAGCGGATTCTGCTGCGTGACGGCGTGCTGACGGTGTGCCCGTCGAC
GCCGGGCTGGTCGATCAACGAGGTGCGCCGACAGTTCGGCGCGGCCAGGCTTGAGCGGGTGCGCGATGACGAGCTCGACG
GCCTGCTGGCCACGGCCTACGCCGATACCGGCAGTGCGGCAGCGGTTGTGGGGGCGGCAGAAAACGAAGTCGACCTAGAT
CGCCTGATGCAGGACATGCCGGAAATCACCGACCTTCTCGACACCCAGGACGGCGCGCCGGTGATCCGCATGATCAACGC
CTTGCTCACGCAGGCGGCGCGGGATGAGGCCAGTGACATTCACATCGAACCGTTCGAAACCCATTCGGTGGTGCGCTACC
GGGTCGACGGCACCCTGCGCGACGTGGTCTCGCCGCGCAAGGCGTTGCATGGTGCGCTGGTGTCGCGGATCAAGATCATG
GCGCAGCTCGATATCGCTGAAAAACGCCTGCCGCAGGACGGCCGCATTGCGTTGCGCGTGGCCGGACGGCCGATCGATAT
TCGTGTTTCCACCGTGCCCACCGGCCATGGCGAGCGAGTGGTGATGCGTCTGCTGGACAAGCAGGCCGGGCGTCTGCATC
TGGAAACGCTGGGCATGGACGCGCAGGTGCTGGCCAAACTCGATCACCTGATCCGCCAGCCCCACGGCATCGTGCTGGTC
ACCGGCCCGACCGGTAGCGGCAAGACCACCAGCCTCTACGCGGCACTGGCGCGACTGGATGCGAGCACCAGTAACATCCT
CACCGTGGAAGACCCGGTGGAATATGACTTGCCGGGCATCAGCCAGATTCAGGTCAACGCGAAGATCGACATGACTTTTG
CATTGGCATTGCGGGCGATTCTGCGGCAGGACCCGGACATCATCATGATCGGTGAAATCCGCGACCTCGAAACCGCGCAG
ATCGCGGTGCAGGCTTCGCTGACCGGTCACCTGGTGCTCGCGACCCTGCACACCAACGACGCGGTGTCGGCGGTCAACCG
CTTGATCGACATGGGCGTCGAACCGTTTCTGCTGGCCTCGTCGATGCTCGGCGTGTTGGCCCAGCGGCTGGTGCGGCGCC
TGTGCAATCAGTGCAAACAGGAAGACCCGGCGACGCCCGGGACCTGGCGCCCGGTCGGTTGTGCGGCGTGCAATCACACC
GGTTACAGCGGCCGGACCGGCATTCACGAATTGTTCTGCATCGACGACGACATCCGCACCCTGATTCACCAAGGGGCAGG
GGAGCAGGCGTTGCGCGCGGCGGCGAGCAAGGCTGGGATGTTCAGCCTGCGTGAGGACGGCGAGCGCTGGATTCGCAGCG
GTGCCACGGCCCCTGAAGAAATCCTTCGCGTGACACGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(107-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

45.254

96.795

0.438
  pilF Neisseria gonorrhoeae MS11

45.414

95.513

0.434
  pilB Vibrio parahaemolyticus RIMD 2210633

46.602

88.034

0.41
  pilB Vibrio cholerae strain A1552

48.101

84.402

0.406
  pilB/pilB1 Synechocystis sp. PCC 6803

40.43

99.359

0.402
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.692

83.333

0.397
  pilB Acinetobacter baylyi ADP1

46.649

82.906

0.387
  pilB Acinetobacter baumannii D1279779

46.154

83.333

0.385
  pilF Thermus thermophilus HB27

47.606

80.342

0.382
  pilB Legionella pneumophila strain ERS1305867

45.244

83.12

0.376
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.627

91.667

0.363

Multiple sequence alignment