Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E8E07_RS16080 Genome accession   NZ_CP040911
Coordinates   3285927..3286727 (-) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter pittii strain AB17H194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3280927..3291727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8E07_RS16060 - 3281993..3282691 (-) 699 WP_150378209.1 YfgM family protein -
  E8E07_RS16065 hisS 3282719..3284011 (-) 1293 WP_150378210.1 histidine--tRNA ligase -
  E8E07_RS16070 ispG 3284008..3285123 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  E8E07_RS16075 - 3285145..3285936 (-) 792 WP_150378211.1 helix-turn-helix domain-containing protein -
  E8E07_RS16080 pilF 3285927..3286727 (-) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  E8E07_RS16085 rlmN 3286742..3287977 (-) 1236 WP_150378212.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  E8E07_RS16090 ndk 3288101..3288532 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  E8E07_RS16095 iscX 3288646..3288846 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  E8E07_RS16100 - 3288946..3289497 (-) 552 WP_005066176.1 phosphatase PAP2 family protein -
  E8E07_RS16105 - 3289522..3290811 (-) 1290 WP_150378250.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=368260 E8E07_RS16080 WP_002114699.1 3285927..3286727(-) (pilF) [Acinetobacter pittii strain AB17H194]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=368260 E8E07_RS16080 WP_002114699.1 3285927..3286727(-) (pilF) [Acinetobacter pittii strain AB17H194]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGATCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744

Multiple sequence alignment