Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GZS14_RS18735 Genome accession   NZ_CP048326
Coordinates   3888340..3888780 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 61     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3883340..3893780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZS14_RS18715 (GZS14_18730) aroP 3884339..3885712 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  GZS14_RS18720 (GZS14_18735) ampE 3885755..3886609 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  GZS14_RS18725 (GZS14_18740) ampD 3886606..3887157 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GZS14_RS18730 (GZS14_18745) nadC 3887245..3888138 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GZS14_RS18735 (GZS14_18750) pilA 3888340..3888780 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  GZS14_RS18740 (GZS14_18755) pilB 3888790..3890175 (+) 1386 WP_001025150.1 type II secretion system protein GspE Machinery gene
  GZS14_RS18745 (GZS14_18760) hofC 3890165..3891367 (+) 1203 WP_000157229.1 protein transport protein HofC -
  GZS14_RS18750 (GZS14_18765) guaC 3891402..3892445 (-) 1044 WP_063115782.1 GMP reductase -
  GZS14_RS23420 - 3892601..3892645 (-) 45 WP_120795372.1 protein YacM -
  GZS14_RS18755 (GZS14_18770) coaE 3892670..3893290 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=367890 GZS14_RS18735 WP_000360904.1 3888340..3888780(+) (pilA) [Escherichia coli strain 61]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=367890 GZS14_RS18735 WP_000360904.1 3888340..3888780(+) (pilA) [Escherichia coli strain 61]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363