Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GZS15_RS18745 Genome accession   NZ_CP048322
Coordinates   3889335..3889775 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 64     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3884335..3894775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZS15_RS18725 (GZS15_18735) aroP 3885334..3886707 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  GZS15_RS18730 (GZS15_18740) ampE 3886750..3887604 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  GZS15_RS18735 (GZS15_18745) ampD 3887601..3888152 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GZS15_RS18740 (GZS15_18750) nadC 3888240..3889133 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GZS15_RS18745 (GZS15_18755) pilA 3889335..3889775 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  GZS15_RS18750 (GZS15_18760) pilB 3889785..3891170 (+) 1386 WP_001025150.1 type II secretion system protein GspE Machinery gene
  GZS15_RS18755 (GZS15_18765) hofC 3891160..3892362 (+) 1203 WP_000157229.1 protein transport protein HofC -
  GZS15_RS18760 (GZS15_18770) guaC 3892397..3893440 (-) 1044 WP_063115782.1 GMP reductase -
  GZS15_RS23420 - 3893596..3893640 (-) 45 WP_120795372.1 protein YacM -
  GZS15_RS18765 (GZS15_18775) coaE 3893665..3894285 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=367808 GZS15_RS18745 WP_000360904.1 3889335..3889775(+) (pilA) [Escherichia coli strain 64]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=367808 GZS15_RS18745 WP_000360904.1 3889335..3889775(+) (pilA) [Escherichia coli strain 64]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363