Detailed information NTG0367752

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: GZS15_RS00860
• Genome accession: NZ_CP048322
• Coordinates: 184839..185822 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain 64
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 179839..190822

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GZS15_RS00845 (GZS15_00845)	dppA	181139..182746 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
GZS15_RS00850 (GZS15_00850)	dppB	182897..183916 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
GZS15_RS00855 (GZS15_00855)	dppC	183926..184828 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
GZS15_RS00860 (GZS15_00860)	amiE	184839..185822 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
GZS15_RS00865 (GZS15_00865)	dppF	185819..186823 (+)	1005	WP_000107024.1	dipeptide ABC transporter ATP-binding subunit DppF	-
GZS15_RS00870 (GZS15_00870)	yhjV	186853..188124 (-)	1272	WP_001307456.1	aromatic amino acid transport family protein	-
GZS15_RS00875 (GZS15_00875)	-	188600..188707 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
GZS15_RS00880 (GZS15_00880)	-	189083..189190 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
GZS15_RS23295	-	189566..189673 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=367752 GZS15_RS00860 WP_001196486.1 184839..185822(+) (amiE) [Escherichia coli strain 64]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=367752 GZS15_RS00860 WP_001196486.1 184839..185822(+) (amiE) [Escherichia coli strain 64]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGTAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGATGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404