Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FEE40_RS05275 Genome accession   NZ_CP040852
Coordinates   1112721..1114091 (+) Length   456 a.a.
NCBI ID   WP_004051773.1    Uniprot ID   A0A2Z4VZF8
Organism   Ligilactobacillus murinus strain V10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1107721..1119091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEE40_RS05250 (FEE40_05235) - 1108913..1109386 (+) 474 Protein_1034 IS30 family transposase -
  FEE40_RS05255 (FEE40_05240) - 1109503..1110837 (-) 1335 WP_004051777.1 C1 family peptidase -
  FEE40_RS05260 (FEE40_05245) rpiA 1110973..1111659 (-) 687 WP_004051776.1 ribose-5-phosphate isomerase RpiA -
  FEE40_RS05265 (FEE40_05250) - 1111684..1111971 (-) 288 WP_039936561.1 GNAT family N-acetyltransferase -
  FEE40_RS05270 (FEE40_05255) - 1112108..1112644 (+) 537 WP_135942004.1 dUTP diphosphatase -
  FEE40_RS05275 (FEE40_05260) radA 1112721..1114091 (+) 1371 WP_004051773.1 DNA repair protein RadA Machinery gene
  FEE40_RS05280 (FEE40_05265) - 1114110..1115219 (+) 1110 WP_135057617.1 PIN/TRAM domain-containing protein -
  FEE40_RS05285 (FEE40_05270) gltX 1115308..1116798 (+) 1491 WP_004051770.1 glutamate--tRNA ligase -
  FEE40_RS05290 (FEE40_05275) cysS 1117044..1118453 (+) 1410 WP_163587789.1 cysteine--tRNA ligase -
  FEE40_RS05295 (FEE40_05280) - 1118455..1118850 (+) 396 WP_004051767.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49264.65 Da        Isoelectric Point: 6.9502

>NTDB_id=367625 FEE40_RS05275 WP_004051773.1 1112721..1114091(+) (radA) [Ligilactobacillus murinus strain V10]
MAKVKTRYVCQNCGYVSPRYLGRCPNCSEWNTLVEEVEQKSAAVKATPRVTLTGTTNAPQKIDTIKISQTPRVDTKNGEL
NRVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSGSLSDQGGKLLYVSGEESANQIKMRAQRLGVSGSEFYLYPETDMGSIL
QNIEDLKPDYVIIDSVQTMQMPEVTSAVGSVAQIREVTAALMQVAKTNGITIFIVGHVTKGGAIAGPKILEHMVDTVLYF
EGDMHRTYRILRAVKNRFGSTNEIGVFEMRDGGLVEVPNPSEIFLEERLSGATGSAVVVSMEGTRPILAEIQALVTPTAF
GNAKRTTTGLDHNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAMSLVSSYRDQQTAPDDCFIGELGLT
GEIRRVTRIDQRIAEAAKLGFKRVFIPQNNLNGLELPTNIDVVGVKTISEALRHVF

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=367625 FEE40_RS05275 WP_004051773.1 1112721..1114091(+) (radA) [Ligilactobacillus murinus strain V10]
ATGGCAAAAGTCAAAACTAGATATGTATGTCAAAATTGTGGCTATGTATCACCACGTTATTTGGGGCGGTGTCCTAACTG
TAGCGAATGGAACACTTTAGTTGAAGAAGTTGAACAAAAAAGTGCAGCGGTTAAAGCTACCCCACGCGTTACTTTGACAG
GAACAACGAATGCCCCACAAAAGATCGATACGATCAAGATCTCACAAACACCTAGAGTCGATACGAAAAATGGTGAATTG
AATCGGGTCTTAGGTGGGGGTGTTGTTCCCGGTTCAATGGTCTTGATCGGGGGCGATCCTGGGATCGGTAAATCAACGTT
ACTTTTACAAGTTTCGGGAAGTTTATCAGATCAAGGGGGAAAGTTGCTCTACGTTTCGGGTGAAGAAAGTGCAAACCAGA
TCAAGATGCGGGCCCAACGCTTGGGAGTCTCAGGCAGTGAATTTTACCTTTACCCTGAGACCGATATGGGCAGTATTTTA
CAAAATATCGAAGACTTAAAACCAGACTATGTCATCATTGACTCAGTTCAAACGATGCAGATGCCAGAAGTTACTTCGGC
GGTCGGAAGTGTAGCGCAGATCCGCGAGGTGACTGCTGCTTTGATGCAAGTTGCTAAGACAAATGGGATCACGATCTTTA
TCGTTGGTCATGTGACCAAAGGTGGCGCGATCGCTGGGCCCAAGATCTTAGAGCATATGGTCGATACGGTGTTGTACTTT
GAAGGTGACATGCATCGCACCTACCGGATCTTACGTGCAGTCAAAAATCGTTTTGGTTCGACTAATGAGATCGGTGTGTT
TGAGATGCGAGATGGCGGGCTAGTTGAAGTCCCTAATCCATCTGAGATCTTCTTAGAAGAGCGTTTAAGTGGTGCGACCG
GTTCGGCTGTCGTGGTCTCAATGGAAGGAACACGGCCGATCTTGGCAGAGATCCAAGCGTTAGTCACCCCAACAGCCTTT
GGCAACGCTAAGCGGACCACGACAGGACTAGATCACAATCGGGTCTCGTTGATCATGGCAGTGCTCGAAAAGCGGGCGGG
GCTACTTTTACAAAATCAAGATGCTTACTTAAAAGCCGCTGGGGGCGTTAAATTAGATGAACCAGCGATCGATCTAGCTA
TCGCGATGAGTTTGGTATCGAGTTATCGTGACCAACAGACCGCACCAGATGACTGTTTTATCGGTGAATTAGGTTTGACC
GGTGAGATCAGACGGGTGACTCGGATCGACCAAAGAATAGCTGAAGCAGCTAAATTAGGCTTTAAACGGGTCTTTATCCC
CCAAAATAATTTAAATGGTCTGGAATTGCCAACTAATATCGATGTAGTTGGCGTTAAGACGATCAGTGAAGCTTTACGGC
ATGTTTTTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(74-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z4VZF8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

68.421

100

0.684
  radA Streptococcus pneumoniae D39

68.421

100

0.684
  radA Streptococcus pneumoniae R6

68.421

100

0.684
  radA Streptococcus pneumoniae TIGR4

68.421

100

0.684
  radA Streptococcus mitis NCTC 12261

68.202

100

0.682
  radA Streptococcus mitis SK321

68.202

100

0.682
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

99.342

0.627

Multiple sequence alignment