Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   SLIT_RS02170 Genome accession   NC_013959
Coordinates   439741..441171 (-) Length   476 a.a.
NCBI ID   WP_013028576.1    Uniprot ID   D5CM63
Organism   Sideroxydans lithotrophicus ES-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 434741..446171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SLIT_RS02135 (Slit_0429) - 435111..435710 (-) 600 WP_013028569.1 flavin prenyltransferase UbiX -
  SLIT_RS02140 (Slit_0430) - 435707..436189 (-) 483 WP_041420732.1 Gx transporter family protein -
  SLIT_RS02145 (Slit_0431) - 436434..436802 (-) 369 WP_013028571.1 NusG domain II-containing protein -
  SLIT_RS02150 (Slit_0432) rapZ 436805..437650 (-) 846 WP_013028572.1 RNase adapter RapZ -
  SLIT_RS02155 (Slit_0433) hprK 437800..438735 (-) 936 WP_013028573.1 HPr(Ser) kinase/phosphatase -
  SLIT_RS02160 (Slit_0434) - 438889..439344 (-) 456 WP_013028574.1 PTS sugar transporter subunit IIA -
  SLIT_RS02165 (Slit_0435) hpf 439401..439724 (-) 324 WP_013028575.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SLIT_RS02170 (Slit_0436) rpoN/rpoN1 439741..441171 (-) 1431 WP_013028576.1 RNA polymerase factor sigma-54 Machinery gene
  SLIT_RS02175 (Slit_0437) lptB 441202..441924 (-) 723 WP_013028577.1 LPS export ABC transporter ATP-binding protein -
  SLIT_RS02180 (Slit_0438) lptA 441921..442508 (-) 588 WP_013028578.1 lipopolysaccharide transport periplasmic protein LptA -
  SLIT_RS02185 (Slit_0439) lptC 442489..443076 (-) 588 WP_013028579.1 LPS export ABC transporter periplasmic protein LptC -
  SLIT_RS02190 (Slit_0440) - 443076..443597 (-) 522 WP_013028580.1 HAD family hydrolase -
  SLIT_RS02195 (Slit_0441) - 443597..444583 (-) 987 WP_041420733.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 476 a.a.        Molecular weight: 53779.77 Da        Isoelectric Point: 4.6533

>NTDB_id=36733 SLIT_RS02170 WP_013028576.1 439741..441171(-) (rpoN/rpoN1) [Sideroxydans lithotrophicus ES-1]
MKHSLQLRQSQQLMLTPQLQQAIKLLQLSTLEINQETARLLDENPFLEREDDNTNQTYSGNSSTDTPAPSSSAESSEPAE
AESRNDSNEWPEPSFSSTSASSPDDEDDGYSEVAAEKPSMREHLLWELNMSQMDPRDKKIVGLLIDALDENAYLSQPLEE
IVELLPPELDITLDDLETALVQLQHLDAPGIGARNLSECLALQLRDMPEDVPGRDLALTIVSQYLDLLAARDFSKLKKTL
RCDDDALRCAQDLIVHLQPKPGSAFEHRAADYVVPDVLVERHGGIWRARLNPEAMPRLRINQVYANILQQRNEKNAQEMA
GQLQEARWFIKNLQQRFETILRVSQAIVERQRQFFEHGDIAMRPLVLREIAEQLELHESTISRVTTQKFMLTPRGIYEFK
YFFGSGLATEAGGACSSTAIRALIKQLVSEEDAKHPLTDSRMSEILAQQGILVARRTVAKYREGMNILPVNLRKSL

Nucleotide


Download         Length: 1431 bp        

>NTDB_id=36733 SLIT_RS02170 WP_013028576.1 439741..441171(-) (rpoN/rpoN1) [Sideroxydans lithotrophicus ES-1]
ATGAAACACTCTCTCCAACTCAGACAATCCCAACAACTGATGCTGACGCCGCAGTTGCAGCAGGCTATCAAGCTGCTGCA
ACTCTCGACGCTGGAGATCAATCAGGAGACTGCGCGCCTGCTGGACGAGAATCCTTTCCTCGAACGCGAGGACGACAATA
CCAACCAGACCTACAGCGGCAACAGCAGCACCGATACCCCCGCCCCATCCAGCAGTGCCGAAAGCAGCGAGCCCGCCGAA
GCGGAATCACGCAACGACAGCAACGAATGGCCGGAACCCTCGTTCTCATCGACCTCCGCTTCCAGCCCGGATGACGAGGA
CGACGGCTATTCGGAGGTCGCTGCCGAAAAACCCAGCATGCGTGAGCACTTGCTCTGGGAATTGAACATGAGCCAGATGG
ATCCGCGCGACAAAAAGATCGTCGGCCTGCTCATCGATGCGCTGGACGAGAACGCCTACCTGTCGCAGCCGCTGGAAGAG
ATCGTCGAGCTGCTGCCGCCGGAACTGGATATCACCCTGGACGACCTGGAAACGGCGCTGGTGCAGCTGCAGCATCTGGA
TGCCCCCGGCATCGGTGCGCGCAACCTGAGCGAATGCCTGGCGCTGCAACTGCGCGACATGCCTGAAGATGTGCCGGGCC
GGGACCTGGCTTTGACCATCGTCAGCCAATATCTGGATCTGCTGGCCGCCCGCGACTTCAGCAAACTGAAAAAGACCCTG
CGCTGCGATGACGATGCCCTGCGCTGCGCCCAGGACCTGATCGTGCATCTGCAACCCAAACCCGGATCTGCTTTCGAGCA
TCGTGCGGCAGACTATGTGGTGCCGGATGTGCTGGTGGAACGCCACGGCGGCATCTGGCGCGCACGCCTGAATCCGGAGG
CCATGCCGCGCCTGCGCATCAATCAGGTGTATGCCAACATCCTGCAGCAGCGCAACGAGAAGAACGCGCAGGAGATGGCC
GGCCAATTGCAGGAAGCGCGCTGGTTCATCAAGAACCTGCAGCAGCGCTTCGAGACCATCCTGCGCGTGTCGCAGGCCAT
CGTCGAGCGCCAGCGCCAGTTCTTCGAGCACGGCGACATCGCCATGCGCCCGCTGGTGCTGCGCGAGATCGCCGAACAGC
TCGAGTTGCACGAGTCCACCATTTCGCGCGTGACTACGCAGAAGTTCATGCTCACCCCGCGCGGCATCTACGAATTCAAG
TACTTCTTCGGCAGCGGTCTGGCCACCGAAGCAGGCGGCGCGTGCTCCTCCACCGCCATCCGCGCACTCATCAAGCAACT
GGTCAGCGAGGAGGATGCCAAGCACCCCCTCACCGACAGTCGCATGTCCGAAATCCTGGCACAGCAGGGCATATTGGTCG
CCCGCCGTACCGTGGCAAAATACCGCGAGGGCATGAACATCCTTCCGGTAAATCTTCGTAAATCACTCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(111-304)

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-474)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D5CM63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.28

100

0.563

Multiple sequence alignment