Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   FHE73_RS20905 Genome accession   NZ_CP040782
Coordinates   4068668..4069102 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain HM-311     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4063668..4074102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FHE73_RS20890 (FHE73_20890) - 4064847..4065404 (+) 558 WP_000062058.1 PadR family transcriptional regulator -
  FHE73_RS20895 (FHE73_20895) metE 4065915..4068203 (+) 2289 WP_001007627.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  FHE73_RS20900 (FHE73_20900) comJ 4068242..4068631 (-) 390 WP_089148309.1 competence protein ComJ -
  FHE73_RS20905 (FHE73_20905) nucA/comI 4068668..4069102 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  FHE73_RS20910 (FHE73_20910) - 4069339..4070088 (+) 750 WP_089148308.1 DUF3967 domain-containing protein -
  FHE73_RS20915 (FHE73_20915) kinB 4070127..4071401 (-) 1275 WP_000420360.1 sporulation sensor histidine kinase KinB -
  FHE73_RS20920 (FHE73_20920) - 4071712..4071912 (+) 201 WP_000929277.1 hypothetical protein -
  FHE73_RS20925 (FHE73_20925) - 4072035..4073198 (+) 1164 WP_089148307.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=367140 FHE73_RS20905 WP_000811494.1 4068668..4069102(-) (nucA/comI) [Bacillus thuringiensis strain HM-311]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=367140 FHE73_RS20905 WP_000811494.1 4068668..4069102(-) (nucA/comI) [Bacillus thuringiensis strain HM-311]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment