Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GWG09_RS18915 Genome accession   NZ_CP048025
Coordinates   3958652..3959092 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain GZEC065     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3953652..3964092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWG09_RS18895 (GWG09_18875) coaE 3954142..3954762 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  GWG09_RS24635 - 3954787..3954831 (+) 45 WP_120795372.1 protein YacM -
  GWG09_RS18900 (GWG09_18880) guaC 3954987..3956030 (+) 1044 WP_001217338.1 GMP reductase -
  GWG09_RS18905 (GWG09_18885) hofC 3956065..3957267 (-) 1203 WP_000157234.1 protein transport protein HofC -
  GWG09_RS18910 (GWG09_18890) pilB 3957257..3958642 (-) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  GWG09_RS18915 (GWG09_18895) pilA 3958652..3959092 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  GWG09_RS18920 (GWG09_18900) nadC 3959295..3960188 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GWG09_RS18925 (GWG09_18905) ampD 3960276..3960827 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GWG09_RS18930 (GWG09_18910) ampE 3960824..3961678 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  GWG09_RS18935 (GWG09_18915) aroP 3961721..3963094 (-) 1374 WP_001331235.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=367124 GWG09_RS18915 WP_000360895.1 3958652..3959092(-) (pilA) [Escherichia coli strain GZEC065]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=367124 GWG09_RS18915 WP_000360895.1 3958652..3959092(-) (pilA) [Escherichia coli strain GZEC065]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363