Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   FHE73_RS05895 Genome accession   NZ_CP040782
Coordinates   1127954..1131679 (+) Length   1241 a.a.
NCBI ID   WP_089149402.1    Uniprot ID   A0A9X6NQU1
Organism   Bacillus thuringiensis strain HM-311     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1122954..1136679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FHE73_RS05880 (FHE73_05880) - 1123112..1123705 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  FHE73_RS05885 (FHE73_05885) lepB 1123762..1124325 (+) 564 WP_000751900.1 signal peptidase I -
  FHE73_RS05890 (FHE73_05890) addB 1124442..1127957 (+) 3516 WP_089149401.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  FHE73_RS05895 (FHE73_05895) addA 1127954..1131679 (+) 3726 WP_089149402.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  FHE73_RS05900 (FHE73_05900) - 1131692..1131979 (+) 288 WP_074594665.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  FHE73_RS05905 (FHE73_05905) gerPF 1132117..1132332 (-) 216 WP_001141566.1 spore germination protein GerPF -
  FHE73_RS05910 (FHE73_05910) - 1132375..1132761 (-) 387 WP_000902323.1 spore germination protein GerPE -
  FHE73_RS05915 (FHE73_05915) gerPD 1132777..1132971 (-) 195 WP_001052806.1 spore germination protein GerPD -
  FHE73_RS05920 (FHE73_05920) gerPC 1132978..1133592 (-) 615 WP_001070760.1 spore germination protein GerPC -
  FHE73_RS05925 (FHE73_05925) gerPB 1133660..1133866 (-) 207 WP_001012516.1 spore germination protein GerPB -
  FHE73_RS05930 (FHE73_05930) gerPA 1133881..1134102 (-) 222 WP_001111188.1 spore germination protein GerPA -
  FHE73_RS05935 (FHE73_05935) - 1134199..1134378 (-) 180 WP_089149403.1 aspartyl-phosphate phosphatase Spo0E family protein -
  FHE73_RS05940 (FHE73_05940) - 1134626..1135525 (+) 900 WP_001213073.1 fumarylacetoacetate hydrolase family protein -
  FHE73_RS05945 (FHE73_05945) - 1135561..1135845 (-) 285 WP_000926865.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142741.08 Da        Isoelectric Point: 4.8313

>NTDB_id=367117 FHE73_RS05895 WP_089149402.1 1127954..1131679(+) (addA) [Bacillus thuringiensis strain HM-311]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADAALLGTLSSAARESWTSLYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEPSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESAGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDVDAELKLGATYPEGEDVAAELLCIQQTEEELIDGEEGSEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELAMLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHIVKIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=367117 FHE73_RS05895 WP_089149402.1 1127954..1131679(+) (addA) [Bacillus thuringiensis strain HM-311]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAGCCAGGCTCTCAGCACATTAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCTATTTCGACGATTCATTCCTTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTGGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACGGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACGTTGCAAGCAGATGCAGCTTTACT
TGGAACGCTATCATCAGCTGCTCGTGAATCGTGGACAAGTTTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGTGATTATAACGAGGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACCAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAATTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGCAGGGAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCCG
AACCAGGACTATTCCTAGGAAAATATAAACGCTTCACACAAGAAGGATTGGGTGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAATTTTATTTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGACTACGATGTTGACGCTGAACTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGCA
TTCAGCAAACAGAAGAAGAATTAATAGACGGTGAAGAAGGTTCGGAAGTAGAAAAGGCACAGCTTGAAGCTCGCCTTATG
GCGCAGCGTATTAAAGCGATGGTCGATTCAGGTTATGAAGTATATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCCCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCAG
TATATGCTGACCTCGCGACTGGTTACTTTGAAGCGACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATCCCGCTTGCAGCTGTACTTCGTTCCCCGATCGTTGGATTAAACGATGAAGAACTTGCAATGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAGGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAACTTACTACAAGGATGGCGTGAATTTGCACGTCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGCGCAAGACAATATGAAGCAACATCATTTAGAGGATTATTCCGTTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGGACGGCGAGGGCCCTCGGTGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTTGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTATTGCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGCAAACGAATTAAATATACAACATTAT
CACAGCTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCATTGACGCGT
GCGAAAGAGAAGTTAATTTTAATTGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATACTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAATACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCGACATCTCATCGCGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGCCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAACAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCCATTGAAAAAGTAATTTCATTCTTCGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGAAAATTCCCAGGCGGATTTGAACAAGCAAAACCAATTTTA
GAAGATCGATATAAAGTACAGCTTTCGTTATATGCAAAAGCACTGGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAATCATATTGTAAAAATTGAAGAATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.488

100

0.537

Multiple sequence alignment