Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GWG09_RS13270 Genome accession   NZ_CP048025
Coordinates   2738145..2739641 (-) Length   498 a.a.
NCBI ID   WP_001040668.1    Uniprot ID   -
Organism   Escherichia coli strain GZEC065     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2733145..2744641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWG09_RS13245 (GWG09_13235) panM 2733367..2733750 (+) 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
  GWG09_RS13250 (GWG09_13240) livJ 2733949..2735052 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  GWG09_RS13255 (GWG09_13245) rpoH 2735324..2736178 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  GWG09_RS13260 (GWG09_13250) ftsX 2736423..2737481 (-) 1059 WP_060618110.1 permease-like cell division protein FtsX -
  GWG09_RS13265 (GWG09_13255) ftsE 2737474..2738142 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  GWG09_RS13270 (GWG09_13260) pilA 2738145..2739641 (-) 1497 WP_001040668.1 signal recognition particle-docking protein FtsY Machinery gene
  GWG09_RS13275 (GWG09_13265) rsmD 2739791..2740387 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  GWG09_RS13280 (GWG09_13270) yhhL 2740377..2740646 (+) 270 WP_001295207.1 DUF1145 family protein -
  GWG09_RS13285 (GWG09_13275) yhhM 2740649..2741008 (-) 360 WP_000042901.1 DUF2500 domain-containing protein -
  GWG09_RS13290 (GWG09_13280) yhhN 2741149..2741775 (+) 627 WP_000964718.1 lysoplasmalogenase -
  GWG09_RS13295 (GWG09_13285) zntA 2741849..2744047 (+) 2199 WP_000106527.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  GWG09_RS13300 (GWG09_13290) tusA 2744149..2744394 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54655.50 Da        Isoelectric Point: 4.1560

>NTDB_id=367096 GWG09_RS13270 WP_001040668.1 2738145..2739641(-) (pilA) [Escherichia coli strain GZEC065]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPVPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=367096 GWG09_RS13270 WP_001040668.1 2738145..2739641(-) (pilA) [Escherichia coli strain GZEC065]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCAGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGTGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCAGAAGAAGAAGCGGCTAAGGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTAGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAAGAAAAACCGACCAAAGAAGGCTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCTGCGAAGG
CGCGTAATGTTGACGTACTGATTGCCGATACCGCCGGACGCTTGCAGAACAAATCGCACCTGATGGAAGAGCTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.571

77.309

0.375