Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FGL66_RS00760 Genome accession   NZ_CP040781
Coordinates   154236..155606 (+) Length   456 a.a.
NCBI ID   WP_180809719.1    Uniprot ID   A0A7H9DIT1
Organism   Staphylococcus sp. 17KM0847     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 149236..160606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL66_RS00740 (FGL66_00735) - 149647..150108 (+) 462 WP_180809715.1 CtsR family transcriptional regulator -
  FGL66_RS00745 (FGL66_00740) - 150129..150650 (+) 522 WP_180809716.1 UvrB/UvrC motif-containing protein -
  FGL66_RS00750 (FGL66_00745) - 150640..151644 (+) 1005 WP_180809717.1 protein arginine kinase -
  FGL66_RS00755 (FGL66_00750) - 151660..154107 (+) 2448 WP_180809718.1 ATP-dependent Clp protease ATP-binding subunit -
  FGL66_RS00760 (FGL66_00755) radA 154236..155606 (+) 1371 WP_180809719.1 DNA repair protein RadA Machinery gene
  FGL66_RS00765 (FGL66_00760) - 155621..156682 (+) 1062 WP_180809720.1 PIN/TRAM domain-containing protein -
  FGL66_RS00770 (FGL66_00765) ispD 156683..157381 (+) 699 WP_180809721.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  FGL66_RS00775 (FGL66_00770) ispF 157374..157850 (+) 477 WP_180809722.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  FGL66_RS00780 (FGL66_00775) gltX 157925..159379 (+) 1455 WP_180809723.1 glutamate--tRNA ligase -
  FGL66_RS00785 (FGL66_00780) cysE 159694..160335 (+) 642 WP_180810439.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50164.70 Da        Isoelectric Point: 7.0876

>NTDB_id=367082 FGL66_RS00760 WP_180809719.1 154236..155606(+) (radA) [Staphylococcus sp. 17KM0847]
MAKKKVTFECTACGYQTPKWMGKCPNCGAWNSMDETIEQKSSSARHGVRAQTTTNTKVQKLDDIKHEQTPRILTESRELN
RVLGGGIVEGSLVLIGGDPGIGKSTLLLQMCAALSKTRKVLYITGEESLNQTKLRADRLEEDASALNVFAETNLEVIHEA
VKKVEPELIVVDSIQTIFHPEISSAPGSVSQVRESTQSLMHIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTHEMGIFEMKQSGLQSVMNPSEMFLEERSSNVPGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVRLTEPAVDLGIIIATATSFKDRAVDGLDCFIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRVIIPQTNIGGWSFPEGIEVVGVTSVHEALKYALKV

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=367082 FGL66_RS00760 WP_180809719.1 154236..155606(+) (radA) [Staphylococcus sp. 17KM0847]
TTGGCCAAAAAGAAGGTGACATTTGAATGTACAGCTTGTGGTTATCAGACACCGAAATGGATGGGAAAATGTCCAAATTG
TGGCGCTTGGAATAGTATGGATGAAACGATTGAGCAAAAGTCATCGAGCGCAAGACACGGTGTTCGTGCTCAAACGACAA
CAAACACTAAAGTCCAGAAGTTAGACGATATTAAACATGAGCAGACGCCACGTATTTTAACAGAAAGTCGAGAACTGAAT
CGTGTATTAGGTGGTGGCATTGTAGAGGGTTCACTTGTACTTATTGGTGGTGATCCGGGTATTGGAAAGTCAACATTGTT
ACTTCAAATGTGTGCTGCACTATCAAAAACACGTAAAGTTTTATATATCACAGGAGAGGAATCATTAAATCAAACGAAGC
TACGTGCGGATCGTCTGGAAGAAGATGCGAGTGCTTTGAATGTTTTTGCTGAAACGAATTTAGAAGTGATTCACGAAGCG
GTCAAAAAAGTTGAGCCGGAGCTTATTGTAGTAGATTCGATTCAAACGATTTTTCATCCGGAAATCAGTTCTGCACCAGG
ATCGGTGTCTCAAGTTCGAGAGAGTACACAAAGTTTGATGCATATTGCTAAACAAATGAATATTGCGACATTTATTGTTG
GTCATGTGACTAAAGAAGGTCAAATTGCTGGTCCACGCTTGCTTGAACATATGGTAGATACAGTACTTTATTTTGAAGGG
GATGAGCATCATGCTTATCGTATTTTACGTGCAGTGAAAAACCGCTTTGGTTCGACACATGAAATGGGTATTTTTGAAAT
GAAACAGTCAGGTCTACAAAGTGTTATGAATCCATCGGAAATGTTTTTAGAGGAGCGTTCGTCTAATGTACCGGGATCCA
CAATAGTTGCAACAATGGAAGGGACGCGCCCACTACTTATTGAAGTACAAGCACTGGTTACACCAACGACATTTAATAAT
CCTCGACGTATGGCAACGGGCATTGATCATAATCGTTTGAGTTTATTAATGGCTGTTTTAGAGAAAAAAGAAGGCTATTT
GTTACAACAACAGGACGCTTATATTAAAGTAGCTGGTGGTGTGAGATTGACAGAGCCTGCTGTTGATTTGGGCATTATTA
TTGCAACGGCAACGAGTTTCAAAGATCGTGCGGTGGATGGTTTAGATTGTTTTATTGGTGAGGTCGGTTTAACAGGTGAG
GTACGTCGGGTATCACGTATTGAGCAACGTGTGCAAGAAGCAGCAAAATTAGGATTCAAACGTGTAATTATTCCGCAAAC
GAATATAGGGGGATGGTCGTTTCCAGAAGGGATTGAAGTAGTCGGTGTAACTTCTGTACATGAGGCATTGAAATATGCAT
TAAAAGTGTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-220)

Subunit ChlI of Mg-chelatase (ChlI)

(347-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H9DIT1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

64.693

100

0.647
  radA Streptococcus pneumoniae Rx1

58.315

98.904

0.577
  radA Streptococcus pneumoniae D39

58.315

98.904

0.577
  radA Streptococcus pneumoniae R6

58.315

98.904

0.577
  radA Streptococcus pneumoniae TIGR4

58.315

98.904

0.577
  radA Streptococcus mitis NCTC 12261

58.315

98.904

0.577
  radA Streptococcus mitis SK321

58.315

98.904

0.577

Multiple sequence alignment