Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FG342_RS14190 Genome accession   NZ_CP040780
Coordinates   2814859..2816223 (-) Length   454 a.a.
NCBI ID   WP_005686111.1    Uniprot ID   A0A2A5L5Z5
Organism   Lacticaseibacillus rhamnosus strain 1.0320     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2809859..2821223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FG342_RS14170 - 2810726..2811112 (-) 387 WP_049170284.1 GntR family transcriptional regulator -
  FG342_RS14175 - 2811305..2812621 (-) 1317 WP_047678758.1 MucBP domain-containing protein -
  FG342_RS14180 - 2812921..2813631 (+) 711 Protein_2543 Rgg/GadR/MutR family transcriptional regulator -
  FG342_RS14185 - 2813723..2814838 (-) 1116 WP_005686113.1 PIN/TRAM domain-containing protein -
  FG342_RS14190 radA 2814859..2816223 (-) 1365 WP_005686111.1 DNA repair protein RadA Machinery gene
  FG342_RS14195 - 2816243..2816782 (-) 540 WP_047678761.1 dUTP diphosphatase -
  FG342_RS14205 - 2817550..2817840 (+) 291 WP_005686109.1 GNAT family N-acetyltransferase -
  FG342_RS14210 - 2818134..2819453 (+) 1320 WP_047677400.1 aminopeptidase C -
  FG342_RS14215 - 2819598..2820944 (+) 1347 WP_047677403.1 C1 family peptidase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49157.58 Da        Isoelectric Point: 8.3128

>NTDB_id=367074 FG342_RS14190 WP_005686111.1 2814859..2816223(-) (radA) [Lacticaseibacillus rhamnosus strain 1.0320]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSTKSAPRQTRTGSKVKPTRMDEVTVTKETRVKTGLAELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLASTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIENVI
DQMQPDYVVIDSVQTMNVPDMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQNGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEIPPTDCFVGEIGLTGEV
RRVNRIEDRVKEAAKLGFKRIFVPRNNLQGWRAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=367074 FG342_RS14190 WP_005686111.1 2814859..2816223(-) (radA) [Lacticaseibacillus rhamnosus strain 1.0320]
ATGGCGAAAGCAAAAACACAATATGTATGTCAAAATTGCGGTTATATTTCGGCGACTTATTTAGGCCGTTGTCCAAACTG
CGGCGGCTGGAATACGCTCGTCGAAGAAACCGTTAGTTCCACTAAATCGGCACCGCGACAAACACGCACCGGCAGCAAGG
TTAAGCCCACACGCATGGACGAGGTCACGGTGACCAAAGAAACCCGGGTTAAAACGGGCTTGGCCGAGTTAAATCGCGTG
CTTGGCGGCGGCGTGGTTCCGGGCTCGTTAGTTCTGATTGGCGGTGATCCCGGCATTGGCAAGTCAACCTTGTTACTGCA
GGTGTCTGGCCAACTAGCTAGCACAGGCGGTACGGTGTTGTATGTTTCAGGTGAAGAAAGCGCCAGCCAGATTAAAATGC
GGGCAGGGCGGCTAGGGGTTGCCAACTCCGGCATGTATTTGTATCCCGAAACCGACATGCCAAGTATCGAAAACGTGATT
GACCAGATGCAACCGGATTATGTGGTGATTGACTCGGTTCAAACCATGAATGTACCGGACATGAATTCGGCAGTCGGCTC
GGTTGCTCAGATTCGGGAAGTCACCGCCGAATTGATGCGAATTGCCAAGTCAAAAGGTGTGACGATTTTTATCGTTGGTC
ACGTGACGAAGGAAGGCGCTATTGCCGGGCCCAAGATTCTTGAGCATATGGTGGACACCGTGCTTTATTTTGAAGGCGAT
ATGCATCATACCTATCGCATCTTAAGGTCGGTCAAGAATCGGTTCGGCTCTACTAACGAAATCGGTATTTTCGAAATGCA
TCAAAATGGTTTACAGGAAGTCGCCAATCCCTCGGAAATCTTCCTGGAAGAACGGCTGGCTGGTGCAACCGGATCGGCGG
TGGTGGTTTCGATGGAAGGTACCCGTCCGATTCTGGTTGAAATCCAGGCCCTCATCAGCCCGACCATGTATGGCAATGCC
AAACGCACGAGCAGCGGCTTGGATCACAACCGCGTGAGCTTGATCATGGCGGTGCTGGAAAAACGTGCCAATTTGATGTT
GCAAAATCAGGACGCTTACTTGAAAGCAACGGGGGGCGTTAAGCTGGATGAGCCGGCGATTGACTTGGCAATGGCAGTGG
CAATTGCCTCGTCATACCGCGACAAGGAAATCCCGCCGACTGATTGTTTTGTCGGTGAAATTGGCTTAACCGGTGAAGTG
CGGCGGGTGAATCGCATTGAAGACCGGGTGAAAGAGGCGGCTAAGTTAGGGTTTAAACGAATTTTTGTGCCGCGAAATAA
CCTGCAAGGCTGGCGTGCACCTAAAGATATTCAGGTTATTGGCGTCACAAGCATTGCCGAAGCGCTGCATAAGGTTTTTA
ATTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

AAA domain (AAA_25)

(67-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A5L5Z5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.433

99.78

0.683
  radA Streptococcus pneumoniae Rx1

68.433

99.78

0.683
  radA Streptococcus pneumoniae D39

68.433

99.78

0.683
  radA Streptococcus pneumoniae R6

68.433

99.78

0.683
  radA Streptococcus pneumoniae TIGR4

68.433

99.78

0.683
  radA Streptococcus mitis SK321

68.212

99.78

0.681
  radA Bacillus subtilis subsp. subtilis str. 168

64.912

100

0.652

Multiple sequence alignment