Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GW576_RS18795 Genome accession   NZ_CP048010
Coordinates   3859266..3859706 (+) Length   146 a.a.
NCBI ID   WP_203045589.1    Uniprot ID   -
Organism   Escherichia coli strain RUT3575     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3854266..3864706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GW576_RS18775 (GW576_18775) aroP 3855264..3856637 (+) 1374 WP_001347572.1 aromatic amino acid transporter AroP -
  GW576_RS18780 (GW576_18780) ampE 3856680..3857534 (-) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  GW576_RS18785 (GW576_18785) ampD 3857531..3858082 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GW576_RS18790 (GW576_18790) nadC 3858170..3859063 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GW576_RS18795 (GW576_18795) pilA 3859266..3859706 (+) 441 WP_203045589.1 prepilin peptidase-dependent pilin Machinery gene
  GW576_RS18800 (GW576_18800) pilB 3859716..3861101 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  GW576_RS18805 (GW576_18805) hofC 3861091..3862293 (+) 1203 WP_000157236.1 protein transport protein HofC -
  GW576_RS18810 (GW576_18810) guaC 3862328..3863371 (-) 1044 WP_001217338.1 GMP reductase -
  GW576_RS18815 - 3863527..3863571 (-) 45 WP_120795372.1 protein YacM -
  GW576_RS18820 (GW576_18815) coaE 3863596..3864216 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15601.72 Da        Isoelectric Point: 4.5219

>NTDB_id=367032 GW576_RS18795 WP_203045589.1 3859266..3859706(+) (pilA) [Escherichia coli strain RUT3575]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=367032 GW576_RS18795 WP_203045589.1 3859266..3859706(+) (pilA) [Escherichia coli strain RUT3575]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

42.742

84.932

0.363