Detailed information NTG0366978

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: GW576_RS01370
• Genome accession: NZ_CP048010
• Coordinates: 214288..215271 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain RUT3575
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 209288..220271

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GW576_RS01355 (GW576_01340)	dppA	210431..212038 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
GW576_RS01360 (GW576_01345)	dppB	212346..213365 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
GW576_RS01365 (GW576_01350)	dppC	213375..214277 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
GW576_RS01370 (GW576_01355)	amiE	214288..215271 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
GW576_RS01375 (GW576_01360)	dppF	215268..216272 (+)	1005	WP_000107031.1	dipeptide ABC transporter ATP-binding subunit DppF	-
GW576_RS01380 (GW576_01365)	yhjV	216302..217573 (-)	1272	WP_001296805.1	aromatic amino acid transport family protein	-
GW576_RS01385 (GW576_01370)	-	218049..218156 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
GW576_RS01390 (GW576_01375)	-	218532..218639 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
GW576_RS01395 (GW576_01380)	-	219015..219122 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
GW576_RS01400 (GW576_01385)	-	219498..219605 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=366978 GW576_RS01370 WP_001196486.1 214288..215271(+) (amiE) [Escherichia coli strain RUT3575]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=366978 GW576_RS01370 WP_001196486.1 214288..215271(+) (amiE) [Escherichia coli strain RUT3575]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATCATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGCCGTCAGCGGGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGTAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGATGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404